乙烯在植物生长发育和应对环境胁迫中起重要作用。EIN2是乙烯信号通路的关键正调组分，其激活乙烯反应的生化机制一直是基础研究领域的难点和热点。近年来，我们的研究发现乙烯诱导EIN2蛋白C端（CEND）发生切割，定位在细胞核中，并通过稳定EIN3蛋白激活乙烯反应。然而，EIN2在细胞核中发挥功能的生化机理仍不清楚。. 为此，本申请拟深入解析EIN2在细胞核中直接激活EIN3的分子机制，可能涉及到了对EIN3二硫键形成以及由此导致蛋白水平和活性的改变。本申请有望揭开EIN2调控乙烯信号的具体生化机制，同时对激素和胁迫交叉响应研究具有指导意义。

Ethylene plays an important role in plant growth and development as well as environmental stresses. EIN2 protein is the key positive component of ethylene signaling pathway and its molecular mechanism on activation of ethylene signaling has been a difficulty and a focus of plant science. In recent years, we have found that ethylene induces the cleavage of EIN2 carboxyl terminal (CEND), which sequentially locates in nucleus and activates ethylene response by stabilizing EIN3 protein. However, the biochemical mechanism of EIN2 CEND activating ethylene signaling in the nucleus remains to be further studied..This study aims to elucidate the molecular mechanism of EIN2 CEND directly activating EIN3 in the nucleus, possibly involving the formation of the disulfide bonds of EIN3 and the resulting alternation of protein level and activity. This study is expected to reveal the biochemical mechanism of ethylene signal in detail, and provides example for the study of hormone and stress cross responses.