疾病最终表现为细胞水平或组织水平上与正常细胞或组织的差异，实现原位、实时、微创式的活体单细胞拉曼光谱检测在疾病诊断上具有重要的应用价值。本项目拟将同轴双波导特种光纤与表面增强拉曼散射技术相结合，设计并制备光纤拉曼探针。该探针可以将传输激发光和探测信号光两个通道集成于一根光纤内，在不增加探针尺寸的前提下，解决探针收集效率和信噪比低等问题，并结合表面增强拉曼散射技术，提高拉曼信号强度。主要达成如下目标：【1】实现液体中探测体积和尺寸在微米量级的微小物质如细胞、微生物、微量液体、痕量物质等探测对象的光纤拉曼测量；【2】实现可以介入活体组织内部，能够实现点接触式的活体组织探测对象的光纤表面增强的拉曼探测。这种光纤拉曼探针能够精确地实现活体细胞拉曼光谱的诊断，使活体细胞和在体细胞拉曼光谱的研究提升到单细胞内部的分子水平。

Disease is finally manifested as the differences from normal cells or tissues at the cell level or organizational level. In situ, real-time and minimally invasive in vivo single cell Raman spectroscopy detection is very important in the diagnosis of disease. This project intends to design and prepare fiber Raman probes by combining special coaxial dual waveguide fibers with surface-enhanced Raman scattering technology. The probe can integrate two channels of transmitted excitation light and detected signal light into one optical fiber, solving the problems of probe collection efficiency and low signal-to-noise ratio without increasing the size of the probe, and by combining with surface-enhanced Raman scattering technology, the Raman signal strength can be improved. The main objectives of this project are as follows:.1. Realize the optical fiber Raman detection for small substance with size order of microns in the liquid, such as cell, microorganism, trace liquid and trace substance; .2. Realize a fiber optic surface enhancement Raman probe that can be inserted into a living tissue to implement point-contact detection. .This kind of optical fiber Raman probe can accurately realize Raman spectroscopy diagnosis, and it may improve the single living cell Raman spectroscopy to the molecular level.