罂粟和梨都具有配子体型自交不亲和性系统，但二者雌蕊S决定因子不同。然而，两者采用相似的信号转导路径抑制不亲和性花粉管生长，并且不亲和性花粉管死亡具有程序性死亡特征。研究发现芸薹属植物孢子体型自交不亲和性反应过程中，乳突细胞微丝骨架发生解聚。微丝骨架解聚往往会引起细胞程序性死亡。为了研究孢子体型自交不亲和性是否通过细胞程序性死亡抑制不亲和性花粉管生长，本项目拟通过检测芸薹属孢子体自交不亲和性反应过程中乳突细胞和花粉（管）中钙离子、活性氧、一氧化氮等信号事件以及微丝骨架、线粒体、核DNA等变化情况，阐明孢子体型自交不亲和性“自我”花粉拒绝机制，明确不同类型自交不亲和性系统是否采用相似路径抑制“自我”花粉管生长。

Although poppy and pear have same gametophytic self-incompatibility, the female S determines between poppy and pear is not same. However, they use a similar signal transduction pathway to inhibit the self/incompatible pollen tube growth, and the death of self/incompatible pollen tube has typical features of programmed cell death (PCD). It is reported that actin cytoskeleton of papilla cells was depolymerized in sporophytic self-incompatibility response of Brassica. Depolymerization of the actin cytoskeleton is adequate to induce PCD. To check whether PCD specifically occur in incompatible pollen tube growth inhibition in Brassica sporophytic self-incompatibility, the changes of some signal factors, i.e., calcium, reactive oxygen species, and nitric oxide, actin cytoskeleton, mitochondria, and nuclear DNA of papilla cells and incompatible pollen grain/tube will be detected in this research project. We believe we can uncover the mechanism of self-pollen tube growth inhibition in sporophytic self-incompatibility, and know whether different self-incompatibility systems use a similar pathway to inhibit self-pollen tube growth.