通过大分子自组装构筑的纳米材料，在结构调控与功能开发等方面引起了科学界的广泛兴趣。鉴于自组装纳米材料在小分子药物传递、基因装载与控释等生物医学研究领域中取得的重要进展，利用大分子组装体制备面向肿瘤免疫治疗的新型纳米材料的研究，将具有重要的研究价值和广阔的应用前景。因此，本研究提出利用大分子自组装体系，将肿瘤相关的Tn糖抗原、核酸佐剂CpG ODN和聚乙烯亚胺（PEI）载体，联合应用于抗肿瘤纳米粒子的制备。这一设计能使CpG-PEI自组装纳米粒子发挥载体和免疫佐剂的双重功能，并充分发挥Tn抗原的免疫激活能力，从而制备多价、多组分、多功能的新型抗肿瘤自组装纳米材料。本研究将利用免疫学实验对纳米材料的抗肿瘤效应进行评估，以期为该纳米材料最终应用于临床治疗提供实验基础。本项目将会为治疗性肿瘤疫苗的设计和开发，启发新思路、提供新策略，并将从理论和实践上加深对肿瘤免疫治疗的认识和理解。

Self-assembled nanomaterials have significant advantage in the regulation of material structure and performance. Due to the self-assembly of nanomaterials research has made significant progress in the field of biomedical research, such as small molecular drug delivery, gene loading and controlled release, the utilization of macromolecular self-assembled nanomaterials for tumor immunotherapy will have considerable significance. Therefore, this study proposes to prepare nanoparticles with anti-tumor property by using tumor-associated carbohydrate Tn antigen, nucleic acid adjuvant CpG ODN and polyethylenimine (PEI) vector based on macromolecular self-assembly system. This design enables the CpG-PEI nanoparticles to play dual function of both carrier and immune adjuvant, and also give full play to the immune activation of Tn antigen. This study aim at design and prepare a novel, multivalent, multi-component, multi-functional self-assembled nanomaterials with potential anti-tumor property. Subsequent study is focus on immunological assessment of this nanomaterial, which provides experimental data for clinical study. This study will inspire new ideas and new strategies for the design and development of therapeutic tumor vaccine.