大黄泻下现“泄热”临床疗效，比照大黄“泻下通便、通下利尿”效应，创新性提出大黄“泄热”之“双通道（结肠与肾）”。AQP2是结肠和肾脏调节水液代谢重要因子，前期研究发现大黄泻下作用可能与降低AQP2表达有关。AVP是中枢发热负调介质，经下丘脑神经垂体束生成释放入血，研究发现AVP中枢和外周给药同样都有退热作用，AVP可通过调节V2受体作用于AQP2，同时AQP2调节可能有促进AVP的作用。然而AVP-V2R-AQP2是否参与体温调节及其机制如何目前尚不清楚，大黄泻下作用伴随的“泄热”疗效是否与AVP-V2R-AQP2有关？大黄不同成分（大黄酸/大黄素/大黄酚等）可否导致泄热部位（结肠/肾脏）差异？本研究拟采用阻断剂及激动剂，通过在体和离体实验，探究大黄是否通过AVP-V2R-AQP2通路发挥泄热作用；采用细胞荧光探针标记技术观测AQP2胞内分布与磷酸化水平，察验大黄效应成分与药效部位的关系。

According to the "purge heat” clinical efficacy due to "diarrhea" effect of rhubarb, and clinical manifestation of "laxative and diuresis”,we innovatively propose the mechanism of rhubarb “purge heat” effect through "dual channel" -colon and kidney.AQP2 is an important factor in the regulation of water and liquid metabolism in the colon and kidney. Previous studies have found that the diarrhea effect of rhubarb may be related to the reduction of AQP2 expression.As a negative mediator of body temperature regulation, AVP is released into the blood via hypothalamus neurohypophysis tract. Studies have found that both central and peripheral administration of AVP have antipyretic effects, and that AVP can act on AQP2 by regulating V2 receptors, and that AQP2 regulation may promote AVP.However, it is not clear whether AVP-V2R-AQP2 Signaling pathway is involved in temperature regulation. What is the mechanism of the " purge heat" effect of rhubarb that related to AVP-V2R-AQP2 Signaling pathway ?Can different components of rhubarb (emodin/emodin/emodin) cause differences in different tissue (colon/kidney)?In this study, blockers and agonists were used to explore the " purge heat" effect of rhubarb that related to AVP-V2R-AQP2 Signaling pathway through in vivo and in vitro experiments.Intracellular fluorescent probe labeling is used to observe AQP2 phosphorylation levels and intracellular distribution.Aims:①Verify the " dual channel " mechanism of rhubarb to purge heat by detecting the AQP2 expression caused by rhubarb and the relation with AVP-V2R-AQP2 Signaling pathway.②Discuss the relationship between rhubarb components and pharmacodynamic sites, and verify the characteristics of "dual channel" to purge heat efficiency of rhubarb.