多囊卵巢综合征（PCOS）是一种最常见的育龄女性内分泌疾病，影响约6%-10%的育龄女性。长链非编码RNA（LncRNAs）近年已成为非编码RNA研究中的热点，已经发现在多种疾病中发挥重要作用。最近，仅有两篇研究初步涉及外周血中LncRNAs与PCOS的关系。在全基因组关联分析阳性区域内，颗粒细胞中是否存在有疾病差异表达LncRNAs，这些LncRNAs通过怎样的机制导致疾病的发生与发展，正是本课题所要聚焦的重要科学问题。.本课题拟以生物信息学挖掘疾病全基因组关联分析中阳性易感区域内的LncRNAs，同时，从疾病及对照组卵泡液颗粒细胞中，筛选出差异表达LncRNA。将差异表达LncRNAs在大样本中进行验证。最后，利用相应细胞系及小鼠，对上述差异LncRNAs进行体外及体内的功能研究，阐明LncRNAs在PCOS疾病中的作用及分子机制。最终为PCOS的发病机制及治疗提供新思路和新方法。

Polycystic Ovary syndrome (PCOS) is one of the common endocrine disorder affecting 6%-10% reproductive women. Recently, Long noncoding RNAs (LncRNAs) has been research highlight in the field of noncoding RNAs. And LncRNAs has been found to be associated with a few important disorders. Recently, there are only two papers，which simply figure out relationship between LncRNAs and PCOS. Therefore, there are two important scientific questions included in this proposal. 1. Are there any LncRNAs in positive region of GWAS study of PCOS. 2. What mechanism of these LncRNAs having effects on etiology and development of disease.. In this study, we are planning to get candidate LncRNAs in the GWAS positive region of PCOS. Secondly, by differentially expressed anaylsis, we want to find disease associated LncRNAs in granulosal cells of PCOS. Then, we will systematically investigate function of corresponding LncRNAs in cell and mouse model. Finally, we want to figure out molecular mechanism of LncRNAs in PCOS and provide insights to disease therapy and studying.