牛源犬新孢子虫感染致孕鼠胎盘的损伤作用及其机制研究

犬新孢子虫是危害养牛业生产的重要病原之一，作为引起孕畜流产、死胎及新生儿运动和神经系统障碍的一种细胞内寄生原虫，对胎盘的损伤机制目前尚不清楚。为确定犬新孢子虫对胎盘的损伤作用，本项目拟以BABL/c小鼠为实验动物，建立牛源犬新孢子虫孕鼠感染模型，应用透射电镜、RNA原位杂交、流式细胞术、SABC及RT2 Profiler PCR Array等技术方法，对孕鼠胎盘组织进行病理学损伤观察、虫体入侵动态观察、细胞凋亡检测、相关细胞因子检测及细胞氧化、凋亡相关蛋白和酶等表达水平检测，以此阐明犬新孢子虫对胎盘组织细胞的损伤作用；采用RIA、ELISA及IHC等方法，检测影响胎盘物质转运、胎盘血流及胎儿生长发育的胎盘激素、生长因子及酶类等各项指标，明确犬新孢子虫对胎盘激素调节功能的影响，进而揭示牛源犬新孢子虫感染致孕鼠胎盘的损伤机制。以期为犬新孢子虫致病作用的研究奠定基础。

Neospora caninum is one of the improtant harmful diseases to the cattle industry. This disease mainly causes the miscarriage of dams, stillbirth, neonatal movement disorders and neurological diseases. However, the mechanism of the injury function is not clear. To determine this, the models of N. caninum infected pregnant rats were established in this project. Transmission electron microscopy, RNA in situ hybridization, Flow cytometry, SABC and RT2 Profiler PCR Array etc. were carried out to observe the pathology of the placentas of pregnant rats, the process of the invasion of N. caninum, cell apoptosis and detect related cytokines and the expression levels of cellular oxidative and apoptopsis related proteins to describe injury function of bovine N. caninum infection to the placentas cell of pregnant rats. By RIA, ELISA and IHC etc. placental hormones, growth factors and enzymes affected material transportion, blood flow of placental and fetal growth and development to define the affection of N. caninum to the regulatory function of placental hormones and thus reveal the injury mechanism of the placentas of pregnant rats infected by N. caninum. This project will settle the solid foundation for the pathogenic role of N. caninum.