miR-542在多氯联苯影响胚胎着床过程中的作用机制与功能研究

胚胎着床失败所致不孕不育是生殖领域亟待解决的重要科学问题，环境因素在其中发挥重要作用，我们前期研究证实环境内分泌干扰物多氯联苯可干扰胚胎着床相关分子表达，引起子宫内膜细胞增殖分化异常，最终导致胚胎着床失败，但具体机制尚未阐明。miR-542是一抑癌基因，与细胞增殖分化、血管生成相关。我们最近研究通过miRNA芯片筛选发现多氯联苯所致胚胎着床失败模型中，miR-542的表达明显下调。但具体机制尚未研究。本项目拟在前期工作的基础上，通过动物模型、细胞模型及临床资料分析，针对胚胎着床这一关键事件环节，以miR-542调控胚胎着床作为研究核心，利用多种生物化学方法，分析PCB影响胚胎着床的微小RNA调控机制，为深入了解 miRNAs 在胚胎着床中的作用以及为某些生殖疾患的诊治奠定坚实的实验基础。

Infertility caused by embryo implantation failure is an important scientific problems in nowadays, in which environmental factors play an important role, we preliminary found that polychlorinated biphenyls (PCBS) produced implantation defect in association with a defective uterine morphology during the implantation period. Alterations in the expression of LIF ESR1、 HOXA10、 ITGB3 could explain, at least in part, the mechanism of effects of PCB118 exposure on endometrial receptivity and embryo implantation. , but the specific mechanism has not yet been elucidated. miR - 542 is a tumor suppressor gene, which is associated with cell apoptosis and inflammation. Our recent research first discoveredpolychlorinated biphenyls (PCBs) could downregulated the expression of miR-542 in CD-1 mice. The project intends to clarify the roles of miR - 542 in implantation failure caused by PCB118.