近红外发射具有检测深度大、生物体背景低等优势，结合稀土发光材料具有发射谱带窄、色纯度高和发光寿命长的特点，本项目提出构建近红外激发/近红外发射的稀土配合物，用于小动物活体水平上特定活性物种的发光成像检测和示踪。拟通过摩尔吸光系数大的近红外染料作为天线来敏化稀土离子，获得近红外激发/近红外发射的稀土配合物；并通过对染料结构的功能性设计，构建具有识别响应能力的近红外稀土配合物探针；以探针发光强度和寿命的变化为检测信号，通过发光成像技术方法在活体水平上实现直接、实时地获得目标活性物种浓度和分布信息。

Near-Infrared (NIR) luminescence shows high penetration depth and low auto-fluorescence from biological samples. Combing with the unique properties of lanthanide-based luminescent materials, such as sharp emission bands, high color purity and long luminescent lifetimes, in this project, NIR sensitized NIR luminescence of lanthanide complexes will be developed for monitoring and imaging some special analytes in living animals. Herein, NIR dyes with high molar absorption coefficient serve as antenna to sensitize the NIR luminescence of lanthanide complexes. In addition, by modifying different functional moieties, NIR probes with specific recognition based on lanthanide complexes can be constructed. Using the NIR luminescence intensity and lifetime as detection signal, the concentration and distribution of the target species in vivo can be obtained directly and real-time through luminescent imaging methods.