弱视是严重影响儿童视功能健康的主要疾病之一，国内患病率为3-3.8%，居视力残疾致病之首位。针灸治疗弱视显示了较好的临床前景。弱视主要受损部位在视皮质，本项目结合前期工作基础和资料，围绕“敏感期内视觉剥夺后视环境功能紊乱导致的视皮层可塑性关键基因表达异常是弱视发生的主要中枢机制”的研究假说，通过复制单眼视觉剥夺弱视模型，借助基因表达序列分析法（SAGE）等高通量基因筛选技术平台，从基因组角度探讨单眼视觉剥夺后视觉可塑性相关基因的表达，试图从视觉可塑性关键期内关键基因的筛选和调节入手，初步揭示视觉可塑期调控的分子机制，并进一步筛选、克隆和鉴定针刺治疗弱视的响应基因，并对其表征蛋白进行表达分析，钓取至少1个针刺治疗弱视响应基因的全长cDNA克隆，对其进行蛋白纯化与活性研究，揭示和阐明针灸治疗弱视的分子机制，也为针刺干预和调节视觉系统可塑性变化的生物学机制提供新的思路和依据。

Amblyopia is one of the major diseases that seriously affect the children’s eyesight, its prevalence rate is 3-3.8% in Chinese population, and also, it is the top list of visual impairment. Acupuncture on Amblyopia has a bright prospect. This program is based on works we have done before, embrace “abnormal expression of Visual cortex plasticity related genes that caused by Disturbance of visual environment after Visual deprivation is the visual central mechanism” research hypothesis. By Duplicating monocular visual deprivation model rats, with the help of serial analysis of gene expression (SAGE) and High-throughput genomics technology, to explore the different expression of Visual plasticity-related gene after monocular visual deprivation from genomic level, also, to reveal the molecular mechanism of critical period regulation of Visual plasticity. Based on this basis, a further study of acupuncture treatment on Amblyopia response gene screening, cloning and identification will be continued, also, take an expression analysis of its Characterization proteins, extract at least 1 whole cDNA of acupuncture treatment on Amblyopia response genes, Protein expression and purification also needed aim at the cloned response cDNA, study on its anti Amblyopia activity, try to illuminate the molecular mechanisms and effect of visual deprivation by treatment of acupuncture, in order to provide some New ideas and evidences of Acupuncture intervention and regulation on Biological mechanism of visual system plasticity changes.