类风湿关节炎（RA）是以关节滑膜炎为主要特征的慢性自身免疫性疾病，具有较高的致残率。lncRNA H19一直被认为只在肿瘤的发生、生长中有调节作用。近来研究表明RA患者的滑膜组织中lncRNA H19表达显著上调，其确切功能和作用机制尚不清楚。本课题拟通过 RT-PCR 技术验证RA、骨关节炎和正常创伤患者的滑膜组织和成纤维样滑膜细胞（FLS）中lncRNA H19的表达水平，随后在RA-FLS中应用siRNA干扰lncRNA H19表达，研究其对RA-FLS的FGF-8、BMP-2表达和炎症因子分泌等生物学行为的影响，同时通过生物信息软件预测、RNA免疫共沉淀和双荧光素酶实验，分析lncRNA H19与miR-130的作用关系，明确lncRNA H19调控RA骨代谢紊乱的作用机制。本课题研究结果将阐明lncRNA H19在RA发生发展中的作用，以期明确RA关节滑膜炎症的发病机制和找到有效的基因治疗靶点。

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by synovitis and high disability rate. Long noncoding RNA H19 has always been considered as a regulatory factor in the growth of tumor only. Recent studies have shown that the expression of lncRNA H19 in synovial tissue of RA patients was significantly upregulated, but the exact function and mechanism remains unclear. In this study, we verified the expression level of lncRNA H19 in synovial tissue and fibroblast-like synoviocytes (FLS) of RA, osteoarthritis and normal trauma patients by RT-PCR. Then we transfected with lncRNA H19 siRNA in RA-FLS to investigate the expression of FGF-8 and BMP-2 and inflammatory cytokines secretion and analyze the interaction between lncRNA H19 and miR-130 by bioinformatics software, RNA immunoprecipitation and dual luciferase assay. Lastly, we explored the mechanism of lncRNA H19 in regulating bone metabolism disorder of RA. The study will clarify the role of lncRNA H19 in the development and progression of RA, which is important for identifying the pathogenesis of synovitis and finding effective target of gene therapy in RA.