抗草甘膦牛筋草EPSPS基因的表达调控机理

我们新近研究结果显示，在喷洒草甘膦之后，抗性牛筋草中草甘膦靶标酶基因EPSPS的mRNA表达量显著上调，而在敏感性牛筋草中该基因表达量变化不明显，推测这可能是牛筋草对草甘膦产生抗药性的原因之一。植物基因的表达是通过其上游调控序列中某些顺式作用元件和细胞核中的转录因子协同调控的，但具体是哪些作用元件和转录因子调控EPSPS基因表达，目前未见报道。因此，本项目拟在前期工作基础之上，通过对抗草甘膦牛筋草EPSPS基因调控序列的克隆、功能分析以及相关转录调控因子的分离与鉴定，探究调控牛筋草EPSPS基因表达的关键顺式元件及转录因子，阐明牛筋草EPSPS基因表达调控机理，并进一步充实转EPSPS基因抗草甘膦作物研究的理论基础。

To study the mechanisms of Eleusine indica resistance to glyphosate, we recently found that after been sprayed glyphosate, the expression of target enzyme gene EPSPS was significantly up-regulated in Glyphosate-resistance biotype of eleusine indica. But the EPSPS gene expression have no significant change in Glyphosate sensitive biotype of Eleusine indica. We speculated that up-regulation of EPSPS gene is one of reasons of Eleusine indica resistant to Glyphosate. Gene expression is co-regulated by some upstream regulatory factors after picked up the glyphosate signal. However, what factors regulate the expression of EPSPS gene have not been reported. Therefore, this project try to analysis the regulatory factors of EPSPS gene expression on the base of previous work. Cloning and analyses the regulatory sequence of EPSPS gene first, and then isolation and identification of the transcription factor regulation from glyphosate-resistance Eleusine indica . This study will help us to understand the key role factors and mechannism of EPSPS gene expression, and it could also enrich the theoretical basis of development of transgenic glyphosate-resistant crops which been transferred of exogenous EPSPS gene.