兔源十二指肠贾第虫集聚体B宿主适应性的基因组特征研究

十二指肠贾第虫（Giardia duodenalis）是一种常见可感染人和多种哺乳动物的人兽共患原虫，对人体健康和畜牧养殖业危害巨大。课题组前期研究发现，兔感染的贾第虫以集聚体B为主，多位点序列分析显示兔源G. duodenalis集聚体B具有宿主适应性特征。本项目拟选择具有代表性的兔源G. duodenalis集聚体B分离株，采用单包囊挑选测序技术获得其全基因组序列。通过与贾第虫其它集聚体（A，B，C，D，E）全基因组序列的群体遗传结构学和比较基因组学研究，筛选与宿主适应性相关的基因，特别是贾第虫-宿主细胞相互作用密切相关的变异特异性表面蛋白（VSPs）基因，为进一步研究与宿主适应性有关的蛋白功能奠定基础。研究结果将推动贾第虫基因分型和分子诊断方法的创新，为贾第虫病的药物治疗和疫苗开发提供潜在靶点或新思路。

Giardia duodenalis is a common zoonotic protozoa that could widely infect humans and many mammals, which is great harmful to human health and animal husbandry. In our previous studies, we found that G. duodenalis assemblage B was the dominant assemblage in rabbits, and the multi-locus sequence typing analysis showed that G. duodenalis assemblage B from rabbits had the characteristics of host adaptability. In this project, the representative isolates of G. duodenalis assemblage B from rabbits were selected, and the whole genomes sequences were obtained by single cyst sequencing technology. Population genetic structure and comparative genomics analysis were carried out by the whole genome sequences with other G. duodenalis assemblages (A, B, C, D, E) for screening of genes related to host adaptability, and in particular on variant specific surface proteins (VSPs) gene closely related to Giardia-host cell interaction, which laid a foundation for further studies of proteins functions related to host adaptation. The results will promote the innovation of genotyping and molecular diagnostic methods for Giardia, and provide potential targets or new ideas for drug therapy and vaccine development of giardiasis.