马铃薯核糖体蛋白StRPS5调控马铃薯免疫应答的机制研究

由致病疫霉引起的晚疫病是马铃薯生产上的毁灭性病害，深入解析马铃薯免疫应答机制对抗病品种选育至关重要。致病疫霉分泌的效应蛋白能靶标植物蛋白，干扰植物的免疫反应实现侵染。目前，有关植物靶标蛋白的研究多集中在泛素化系统、免疫相关蛋白酶等几个方面，是否有新的植物免疫应答机制还有待解析。申请人前期研究发现马铃薯核糖体蛋白StRPS5能够与致病疫霉效应蛋白Pi16275互作，利用VIGS研究发现RPS5烟草沉默株系对致病疫霉的抗性明显降低。鉴于有关植物核糖体蛋白参与免疫应答的研究较少且缺乏分子机制解析，本项目拟以StRPS5为切入点，解析StRPS5与Pi16275的互作机制，分析StRPS5基因敲除/沉默、过表达对马铃薯抗病性的影响，进而阐明该基因调控马铃薯免疫应答的分子机制。本项目的顺利实施将揭示核糖体蛋白调控植物免疫应答的分子机制，进一步完善植物免疫应答研究，为马铃薯抗病品种的选育提供理论指导。

Late blight caused by Phytophthora infestans is a devastating disease in potato production. In-depth analysis of the immune response mechanism of potatoe is very important to the selection and breeding of potato disease resistant varieties. The effector protein secreted by P. infestans can target plant proteins to interfere with plant immune system to achieve infection. At present, the research on plant immune response mostly focuses on ubiquitination system and immune-related proteases etc. Whether there is a new immune response mechanism in plants remains to be resolved. Our previous study found that the potato ribosomal protein StRPS5 could interact with the P. infestans effector Pi16275. Using VIGS technology, it was found that the RPS5 silent tobaccos have greatly reduced resistance to P. infestans. In view of the fact that there are few studies on plant ribosomal proteins involved in immune response and these studies often lack molecular mechanism analysis，this project intends to use StRPS5 as an entry point to analyze the interaction mechanism between StRPS5 and Pi16275, and analyze the effect of knockout/silence and overexpression of StRPS5 gene on potato resistance to late blight, and then clarify the molecular mechanism of StRPS5 regulating potato immune response. The successful implementation of this project will reveal the molecular mechanism of ribosomal protein regulating plant immune response, further improve the research on plant immunity mechanism, and provide theoretical guidance for the selection and breeding of disease-resistant potato varieties.