PM1及主要内载组分对儿童注意缺陷多动障碍的影响及其表观遗传机制研究

作为PM2.5的重要组成部分，PM1对人群健康危害研究备受关注；最新研究报道PM1可穿透血脑屏障进入脑脊液从而导致神经功能障碍。在前期工作中，申请人基于大规模人群研究发现PM1与儿童ADHD的患病存在显著的暴露—反应关联，表明PM1对儿童ADHD的发生发展具有潜在影响，但内在机理并不清楚。本研究拟开展为期3年的前瞻性巢式病例对照研究，采集PM1并解析内载组分，结合动物吸入模型，利用转录组测序和基因组DNA甲基化谱高通量检测技术，筛查特异、可调控的表观遗传生物学标志，识别与ADHD相关的关键靶点，阐明其潜在的可能致病机制，从而对全面认识大气PM1的毒性健康危害效应和优化大气污染防控具有重要的公共卫生学意义。

Being important component of PM2.5, the effects of PM1 on human health have been a matter of great concern. Recently, it has been reported that PM1 can lead to nerve dysfunction via directly penetrating the blood-brain barrier. In the previous study, we found an association between PM1 and children ADHD based on population-based investigation, which indicated the potential effect of PM1 on children ADHD. However, the mechanisms are still unclear. With a 3-year prospective nested case-control study and an animal inhaled model, using transcriptomics sequencing and high-through detection of Genome DNA Methylation Spectrum, the present study tries to screen special epigenetic biomarkers and identify the key points of ADHD to explore the potential mechanisms of ADHD. Thus, of great public health concern, the present study will be useful to clarify the health effects of PM1 and prevent and control air pollution.