特异性抗体通过诱导Sca-1+心肌干细胞富集促进心肌再生在大鼠心肌梗死模型中的实验研究.

以往观点认为心肌细胞不可再生性影响心梗后的心肌修复，近期研究发现心肌组织中存在具有分化能力的成体干细胞，其中Sca-1+心肌干细胞存在比例多，且分化能力强，研究证实Sca-1+心肌干细胞的治疗可修复重生梗死心肌。在前期实验中，我们利用Sca-1特异性抗体制备胶原材料，通过补片模型修复小鼠梗死心肌区域，发现Sca-1特异性抗体胶原可以显著提高小鼠心肌梗死后的心功能和心肌再生能力。因此，我们将建立大鼠心肌梗死模型，自制交联抗Sca-1单克隆抗体的胶原补片，进一步验证利用抗体诱导Sca-1+心肌干细胞富集可促进心肌再生。我们将从细胞水平证实自制的交联抗Sca-1单克隆抗体的胶原材料可以富集Sca-1+心肌干细胞，并且此干细胞具有多向分化能力，并可分化为功能心肌细胞。其次，我们用此胶原补片修复大鼠心肌梗死区域，随访心功能、提取心肌细胞检测是否存在体内Sca-1+心肌干细胞富集和和分化为功能心肌细胞

The non-regeneration characteristic of cardiomyocytes influence myocardial repair, recent research have found that there are somatic stem cells in cardiac tissue, and as one of them, Sca-1+ cardiac stem cell account for a large proportion, and in vitro and in vivo researches have proved myocardial regeneration results. In our cases, we crosslink monoclonal antibody of Sca-1 with collagen scaffold, which inducing the Sca-1+ cells from autologous heart contacting with monoclonal antibody in target location, and improving myocardial regeneration. Thus, we plan to establish rat myocardial infarction model, and manufacture the collagen scaffold cross-linked with monoclonal antibody of Sca-1 in the further research. First, We will confirm that the collagen scaffold cross-linked with monoclonal antibody of Sca-1 can enrich Sca-1+ cardiac stem cells and the stem cells have multipotent differentiation characteristic and differentiate into functional cardiomyocytes. Then we will repair the rat infarcted dozen with the collagen scaffold, and detect the positive result of Sca-1+ cardiac stem cells enrichment and differentiation into functional cardiomyocytes through the methods of follow-up cardiac function detecting and extract the cardiomyocytes from rat model.