外源苯并噻二唑（BTH）调控百合花香单萜类物质代谢的机制解析

芳香是花卉重要品质性状之一。东方百合花香浓郁，单萜类物质是其主要花香成分。在室内小空间应用时，百合浓郁的花香常使人感觉不适。苯并噻二唑（BTH）是常见的植物激活剂，对植物萜类代谢也有显著调控作用。本项目前期研究表明施用BTH显著减弱了百合的花香（单萜类为主），但BTH影响单萜类物质合成的转录调控和关键酶活性的机制尚不清楚。本项目在明确百合主要挥发物及其合成关键酶基因的基础上，基于转录组数据，筛选差异表达的转录因子与结构基因并验证其功能，明确BTH调控百合花香的分子生物学机理；利用酶动力学研究以及酶抑制物质的分离与鉴定，明确BTH对单萜类物质代谢关键酶活性的影响；结合代谢组学数据，分析BTH对百合单萜代谢中间产物的影响，最终阐明BTH对百合单萜类物质代谢的调控机制。项目实施可以丰富植物萜类代谢调控的相关理论，也为研制高效花香调节剂，改良浓香型百合花香性状奠定理论基础。

Floral scent is one of the important quality traits of ornamental plants. Oriental lily hybrids are rich in fragrances, in which monoterpenoids are the main floral components. When used in small indoor spaces, the rich floral fragrance of lilies often makes people feel uncomfortable. As a common plant activator, benzothiadiazole (BTH) proved to be an effective regulator of plant volatiles as well. Previous studies in this project showed that the application of BTH significantly attenuated the floral aroma of lilies (mainly monoterpenoids), but the mechanism by which BTH affects the transcriptional regulation and key enzyme activities of monoterpenoid synthesis is unclear. Based on the clarification of main volatiles of lilies and key enzymes genes of its synthesis, this project screens differentially expressed transcription factors and structural genes in terms of transcriptome data and verifies their functions of them, and clarifies the molecular biological mechanism of BTH regulating lily flower aroma. Enzyme kinetics continues with isolation and identification of enzyme inhibitors will revel changes of enzyme activities after BTH treatment. Metabolomics will be the key to uncover the changes of metabolites in lilies by BTH treatment. The implementation of this project can explore the mechanism of exogenous BTH regulation of lilies volatiles synthesis, enrich the theory of plant terpenes metabolism and regulation, and lay a theoretical foundation for the development of high-efficiency floral regulators and the improvement of the fragrant scent of lily.