RNA解旋酶SOT1调控水稻高温响应的分子机理研究

全球环境气候变化带来的频繁高温胁迫是植物面临的主要的非生物胁迫之一，对农业生产和粮食安全造成严重威胁。核糖体RNA(rRNA)是核糖体的重要组成部分，在核糖体生物合成和蛋白翻译过程中发挥着重要作用。rRNA加工成熟过程与核糖体生物合成紧密联系，而核糖体组装正常与否对生物体的健康至关重要。前期研究发现温度可通过影响rRNA的加工和核糖体组装进而参与植物的生长发育过程。本研究以水稻为研究材料，综合采用遗传学、生物化学、分子生物学、蛋白质组学、基因组学和生物信息学等方法，以参与调控水稻高温响应的RNA解旋酶SOT1为切入点，研究其在核糖体RNA加工代谢及蛋白质翻译过程中的调控网络和作用机理，阐明其在植物生长发育和温度响应中的功能和分子机理。

Frequent high temperature stress caused by global environmental climate change is one of the main abiotic stresses for plants, which poses a serious threat to agricultural production and food security. Ribosomal RNA (rRNA) is the fundamental component in ribosome, and plays essential roles in ribosome biogenesis and translation. The processing of rRNAs is tightly coupled with ribosome biogenesis, and abnormal processes will disturb the organism development. Previous study showed that temperature impacts plant development through affecting rRNA processing and ribosome assembly. In this project, by using multiple methods, we will dissect the regulatory network and molecular mechanism of RNA helicase SOT1 in pre-rRNA processing and protein translation, and elucidate the role of SOT1 in plant development in response to temperature alterations.