基于Axin-Wnt信号调节基因探讨苗药金乌健骨方治疗膝骨关节炎的分子机制

轴蛋白(Axin)作为一个新发现的构架蛋白，通过与Wnt信号相关调节基因结合，下调β-连环蛋白（β-catenin）的表达，介导骨关节炎（OA）软骨细胞的成熟、增殖、分化及凋亡。据最新国内外研究证实，Wnt/β-catenin信号通路在膝骨关节炎（KOA）软骨细胞的分化、增殖和凋亡中发挥重要作用。KOA属于苗医学“冷骨风”范畴，根据苗医学“壅塞为病，通达为康”的理论，治疗宜“通筋散血”，在此理论指导下以苗药为主组成金乌健骨方，经前期临床和实验研究取得了良好疗效。因此，课题组提出苗药金乌健骨方可能通过调控Axin-Wnt信号相关基因维持KOA软骨细胞增殖与凋亡的平衡，从而修复关节软骨的科学假说。并在前期研究基础上，构建KOA动物模型和人软骨细胞模型，以Wnt/β-catenin信号通路为靶向，Axin作为该通路的主要调节因子，探讨金乌健骨方治疗KOA的分子机制，具有重要的科学意义和应用前景

Axin is a new found scaffold proteins, it can combine with Wnt signal to reduce the expression of β-catenin and mediate the maturation, proliferation, differentiation and apoptosis ofchondrocyte in osteoarthritis (OA). According to the latest study at home and abroad, Wnt/β-catenin signal pathway play an important role in the differentiation、proliferation and apoptosis of chondrocyte in knee osteoarthritis(KOA). KOA belongs to Lenggufeng in Miao medicine, beneath thetheory that congestion turns into disease and patency becomes healthy, we mainly use Miao medicine to compose Jinwujiangufang which has the effect of clearing sinew and scattering blood stasis. Through the early clinical and experimental study, we found the treating effect ofprescription is well, so we come up with a hypothesis that Jinwujiangufang could balance proliferation andproliferation of chondrocyte through regulate Axin-Wntsignal related gene, and then repair arthrosis. So based onWnt/β-catenin signal pathway and Axin regulate gene, we plan to build up KOA animal model and chondrocyte model of human beings to probe the molecular mechanism of using Jinwujiangufang from Miao medicine to treating osteoarthritis.