GbGELP113基因参与水杨酸信号通路响应棉花盐胁迫机制研究

GDSL脂肪酶（GELP）是一类水解酶，广泛参与植物逆境胁迫等生理活动。前期2份海岛棉材料的盐胁迫转录组及蛋白组研究表明，GELP基因家族的多个成员不仅参与盐胁迫过程，还参与响应水杨酸（SA）生物过程；SA合成途径中的关键基因显著差异表达，SA调控非生物胁迫信号通路上多个基因显著差异表达。过表达GbGELP113基因增强了拟南芥耐盐性；外源水杨酸能够缓解棉花的盐胁迫。基于以上研究，推测GbGELP113通过水杨酸信号通路参与棉花对盐胁迫的响应。本项目拟在盐、水杨酸处理下，开展GbGELP113基因的启动子顺式作用元件和互作蛋白研究；以不同处理的GbGELP113过表达及敲除转基因株系为材料，测定SA含量、抗氧化酶系统和抗坏血酸-谷胱甘肽系统等酶活力，分析SA合成及调控非生物胁迫信号通路相关基因的表达丰度，进行脂质组学研究，探究GbGELP113基因参与水杨酸信号通路响应棉花盐胁迫的机制。

GDSL-type esterases/lipases(GELP) were a type of lipid hydrolysis enzymes widely involved in physiological activities such as plant stress. The transcriptome and proteome analysis of two Gossypium barbadense materials showed that more than one member of GELP gene family not only participated in the process of salt stress, but also in the biological process of salicylic acid (SA), and the key genes in SA synthesis pathway and the reported abiotic stress signal pathway regulated by SA were significantly differentially expressed. In Arabidopsis, overexpression of GbGELP113 gene increased salt tolerance in transgenic plants. SA could alleviate salt stress of cotton. Based on the above research, GbGELP113 was speculated to be involved in the response to cotton salt stress by the signal pathway of salicylic acid. This project will intend to carry out the research on promoter cis elements and interacting proteins of GbGELP113 gene under salt and salicylic acid treatment. The SA content and the activity of antioxidant enzyme system and ascorbate-glutathione system will be determined, the expression abundance of gene in the SA synthesis and regulation of abiotic stress signal pathway, and lipidomics will be studied in the GbGELP113 overexpression and knockout transgenic lines under salt and salicylic acid treatment. The mechanism of GbGELP113 gene involved in salicylic acid response to salt stress in cotton will be explored.