压电/介电复合驱控的肿瘤细胞机-电特性同步测试研究

肿瘤细胞生物物理特性是恶性肿瘤早期诊断的有效病理标志，但当前主流的独立测试方法仍面临参量受限、辨识率不足的瓶颈问题，临床应用受到制约。项目提出构建一种基于压电/介电主动加载方式的肿瘤细胞机-电特性同步测试新方法，实现微尺度下细胞机-电特性多参数同步测量与精准表征。内容包括：建立基于微观三维结构的细胞机-电特性模型，探索细胞机-电特性耦合作用机理；设计优化压电驱动柔顺结构，获取细胞典型机械特性曲线与机械特征参量（弹性模量、泊松比、剪切模量、变形度等）；利用微尺度电极，基于生物介电谱获取细胞典型电学特性曲线与电学特征参量（阻抗、迟滞频率、导电率、介电常数、细胞膜比电容等）；最终，结合细胞机-电特性模型与微流体通道，构建压电/介电复合驱控的肿瘤细胞机-电特性同步测试新方法。本项目有望提高肿瘤细胞辨识率水平、破解细胞生物物理特性临床应用瓶颈难题，为恶性肿瘤早期诊断这一重大民生需求提供新思路。

The biophysical characteristic of tumor cells is an effective pathological marker for the early diagnosis of tumors. However, most of the current testing techniques of cell characteristics are based on independent testing methods, which are still faced with the problems of limited parameters and insufficient recognition rate. This project proposes to construct a novel synchronous measurement for the multiple parameters of cell’s mechanical-electrical characteristics driven by piezoelectric/dielectric techniques to achieve multiple parameters measurement under micro-scale. The research content includes: establishing the mechanical-electrical model of cells based on the micro 3D structure, and exploring the coupling principle of cell’s mechanical-electrical properties; obtaining typical mechanical test curves and mechanical characteristic parameters (elastic modulus, Poisson's ratio, shear modulus, deformation degree, etc.) by the design and optimization of piezoelectric flexure mechanisms; typical electrical characteristic curves and electrical characteristic parameters (impedance, relaxation frequency, conductivity, dielectric constant, cell membrane specific capacitance, etc.) are achieved by utilizing micro-scale biological dielectric spectroscopy with micro electrodes; finally, a novel synchronous measurement method for the mechanical-electrical properties of tumor cells is established by piezoelectric/dielectric techniques. This research is expected to improve the level of tumor cell recognition rate, solve the clinic application problem and provide a new thought for the major livelihood needs of early diagnosis of malignant tumors.