人上胚层退出Naïve多能性的分子机制研究

早期人类胚胎着床后的发育包括一系列复杂的形态发生事件。对这些过程缺乏精确的时空控制会导致妊娠失败。由于胚胎发育在物种间有明显区别，直接利用人胚胎研究着床后发育就显得很有必要。人胚胎着床后的发育过程与上胚层（EPI）的发育密切相关。随着胚胎着床，上胚层（EPI）经历形态发生、前后轴形成并在原条处起始胚层的形成，其多能性也从最初的原始态（naïve）退出并过渡到始发态（primed）。naïve多能性的退出起始是EPI发育的最早事件,但其分子机制还不清楚。在本研究中，我们将结合我们之前报道的人胚胎3D培养系统以及单细胞组学测序的方法。将已经获得的单细胞数据（scATAC-seq 和scRNA-seq）进行整合分析，研究naïve多能性退出的分子机制以及滋养层在其中所起的作用。

Human early post-implantation development involves a series of complex morphogenetic events. The normal development of embryos need precise spatial and temporal control over these processes. As the embryogenesis diverge significantly among species. it becomes necessary to investigate post-implantation development on the human embryo directly. The development process of human post-implantation is closely related to the epiblast (EPI). As embryo implants, epiblast undergoes morphological occurrence, establishment of the anterior-posterior axis and the formation of the germ layers at the primitive streak，with transitions from a previously state of naïve pluripotency toward a state of primed pluripotency. The initiate of naïve pluripotency exit is the earliest event of EPI development, but the underlying molecular mechanism is still unclear. In this study, we will union our previously reported human embryo 3D culture system and single-cell omics sequencing methods, study the molecular mechanisms underlying naïve pluripotency exit and the role of trophoblasts during the process through integrated analysis of the obtained single-cell data (scATAC-seq and scRNA-seq).