足大趾（肝经大敦穴）与生殖器官关联的脑“交互联系”

足厥阴肝经起于足大趾，沿下肢上行与前阴联系，体现了体表与体表联系这一重要生命现象，然而其科学内涵尚不清楚。神经科学研究表明，在大脑皮层躯体感觉定位图上，足大趾（肝经起点大敦穴）与生殖器相互毗邻，病理状态下会发生功能重组。而初级感觉皮层的功能重组程度与外周的伤害性刺激强度呈正相关。临床证据显示针刺可以调节大脑皮层的功能重组。因此，本研究拟采用嗜神经病毒示踪、电生理及双光子钙成像的方法从形态和功能两方面探讨大敦穴区与生殖器区在初级感觉皮层功能重组的基础。并评价针刺大敦穴对诱发性前庭痛模型小鼠的干预效应及初级感觉皮层神经元活动的影响，探讨调节脑功能重组在针刺改善诱发性前庭痛小鼠疼痛症状中的作用。从而阐述初级感觉皮层中大敦穴与生殖系统的毗邻关系及其相互联系对机体生理病理状态以及针灸干预作用的影响，为揭示经络学说体表——体表联系的科学内涵提供翔实的科学依据。

The liver meridian, starting from the hallux and connecting with the genitals along the lower limbs, reflects the vital phenomenon of the somato-somatic relationship. The cortical neighboring relationship and functional reorganization under pathological conditions between hallux (Dadun) and genitalia have been revealed in neuroscience research. The degree of functional reorganization of the primary somatosensory cortex (S1) is positively correlated with the intensity of peripheral noxious stimuli. Clinical evidence shows that acupuncture can regulate the functional reorganization of the cerebral cortex. Thus, in this study, we will perform neurotropic virus tracing, electrophysiological recording and two-photon calcium imaging methods to explore the basis of functional reorganization between Dadun acupoint area and genital area in S1 from morphological and functional aspects. The interventional effect and influence on S1 neuronal activity of acupuncture at Dadun will be evaluated to investigate the role of acupuncture regulating brain functional reorganization in alleviating pain in mice with Provoked vestibulodynia. It’s our purpose to elucidate the influence of adjacency and interaction between representative areas of Dadun and reproductive system in S1 on physiological and pathological states as well as the therapeutic effects of acupuncture, which will provide detailed scientific evidence to reveal the scientific connotation of somato-somatic relationship of meridian theory.