中华乌塘鳢中雄激素核受体Arα调控性信息素受体Ep1基因表达的协同因子的鉴定与功能研究

海洋洞穴鱼类中华乌塘鳢（Bostrychus sinensis）雌雄鱼通过性信息素协调生殖同步。雄鱼通过嗅觉器官（嗅囊）感受雌鱼释放的性信息素（如：前列腺素PGE2)。雄鱼嗅囊对性信息素PGE2的敏感性受到雄激素的调控，雄激素通过核受体Arα上调嗅囊中PGE2受体Ep1的表达，增强雄鱼嗅囊对性信息素PGE2的敏感性。有趣的是，嗅囊中Arα对Ep1的上调作用，在精巢处于发育早中期（Ⅰ、Ⅱ和Ⅲ期）的雄鱼中无效，而在精巢处于发育后期和成熟期（Ⅳ和Ⅴ期）的雄鱼中有效，提示着该调控作用依赖某个（类）协同因子的参与，亟待深入揭示。基于此，本研究拟鉴定该协同因子、明确该协同因子的功能、验证该协同因子协助Arα调控Ep1的表达。研究成果可进一步阐明雄激素调控嗅囊对性信息素PGE2敏感性的分子机制，丰富和完善鱼类性信息素研究的理论基础。

In the burrow-living fish Chinese black sleeper (Bostrychus sinensis), females and males employ the sex pheromone PGE2 to synchronize their ovulation and spermiation. The olfactory sensitivity to the sex pheromone PGE2 is modulated by androgens. Androgens elevate the expression of Ep1 via Arα, and consequently increase the olfactory sensitivity to PGE2. Interestingly, the regulatory effects of Arα on Ep1 are valid only in the males at stages Ⅳ or Ⅴ, suggesting that a key cofactor is required in the regulation. This study aims at identifying this cofactor, determining its function and proving its importance in the regulation of Arα on Ep1. The findings of this study would further elucidate the molecular mechanism whereby androgens regulate the olfactory sensitivity to PGE2, and enrich our basic knowledge on teleost pheromones.