凡纳滨对虾干扰素调节因子（LvIRF）启动子区（CT）n微卫星多态对抗病毒性状的调控机制研究

病害频发是制约我国对虾养殖产业健康、可持续发展的主要瓶颈。病害发生受病原感染、环境胁迫以及对虾自身免疫力的共同影响，因此选育高抗病性状的对虾品种是减少病害发生的有效途径之一。凡纳滨对虾干扰素调节因子（LvIRF）是对虾干扰素途径的一个关键转录因子。前期工作发现，LvIRF的启动子区存在一个微卫星序列，命名为（CT）n-LvIRF微卫星，该微卫星多态能够调控LvIRF基因的表达，并且影响对虾的抗WSSV性状，但作用机制尚不清楚。本项目在前期工作基础上，针对（CT）n-LvIRF微卫星多态对LvIRF表达的调控机制进行研究，揭示（CT）n-LvIRF微卫星多态调控对虾抗病毒性状的机制。本项目的研究一方面可揭示启动子区微卫星调控基因表达的分子机制，另一方面为以功能基因（LvIRF）为靶标的对虾分子育种奠定基础。

Disease is the main bottleneck restricted the development of shrimp industry in China, it is affected by pathogen infection, environmental stress and the immunity of shrimp. Therefore, breeding shrimp with high disease resistance is one of the effective ways to reduce the occurrence of diseases. Litopenaeus vannamei interferon regulatory factor (LvIRF) is an important transcription factor of the shrimp interferon pathway. The microsatellite named (CT)n-LvIRF, which located in the promoter region of LvIRF, could regulate the expression of LvIRF and affect the antiviral traits of shrimp, but the mechanism of action is still unclear. Based on the previous work, this project focused on the the regulation mechanism of (CT)n-LvIRF microsatellite the expression of LvIRF, aims to reveal the mechanism of (CT)n-LvIRF microsatellite regulated shrimp antiviral traits. On the one hand, this project could reveal the molecular mechanism of microsatellite, which located in the promoter region, regulated genes expression, and on the other hand, laid the foundation for molecular breeding of shrimp with LvIRF as a marker.