噪声性听力损失（Noise-Induced Hearing Loss，NIHL）是我国最常见的职业危害因素之一，占我国每年新增职业病的1/6。成纤维细胞生长因子1（FGF-1）在内耳的发育和耳蜗组织的损伤修复中发挥着重要的作用。我们前期已经建立了耳蜗毛细胞模型和噪声性听力损失易感人群筛选方法，筛选了与听力损失有关的目标miRNA及靶基因SNP位点。本研究将通过流行病学调查、动物实验以及细胞实验等方式，采用免疫组化、ABR阈值测定、全耳蜗灌注、流式细胞术等技术，研究FGF-1在NIHL和毛细胞氧化损伤与凋亡过程中表达变化与作用机制、FGF-1 3'UTR区SNP位点多态性受miR-133a调控及对毛细胞氧化损伤功能的影响；检测FGF-1促分裂序列缺失在毛细胞氧化损伤与凋亡中的保护作用。本课题有助于阐明FGF-1在听力损失过程中的变化趋势及作用机制，为miRNA-SNP功能研究提供新的思路。

Noise-Induced Hearing Loss（NIHL）is one of the most common occupational hazards and one-sixth of the increased occupational disease every year. Fibroblast growth factor 1 (FGF-1) could expression in all the cells from three embryonic germ layers, and it play a critical role in the development of the inner ear and repair the damaged cochlear tissue. We study the expression and function of FGF-1 in the NIHL and hair cells oxidative damage and apoptosis process, and investigate the function influencing of the FGF-1 3'UTR area SNP regulated by miR-133a and hair cells oxidative damage, and detection the protect effect of FGF-1 in hair cells oxidative damage and apoptosis process. The method related to immunohistochemical, real time PCR, western-blot, abortus Bang reaction, the whole cochlea perfusion and Flow cytometry and so on. Our study provide new insights into the mechanisms of FGF-1 in the hearing loss, which could lead to novel method to study the function of miRNA-SNP.