以有毒微囊藻为代表的蓝藻水华频发暴发，严重危害水环境。混合营养型原生动物棕鞭毛虫Ochromonas能高效率清除有毒微囊藻并快速降解藻毒素，把有害藻类通过食物链化害为利从而疏通能量通道。但目前对棕鞭毛虫清除有毒微囊藻如何响应多样化生态环境尚缺乏系统认识，其如何降解藻毒素的机制也是亟待揭示的基础科学问题。本申请拟把棕鞭毛虫降解藻毒素能力置于多样化的生物和非生物因素中进行研究，明确影响棕鞭毛虫清除微囊藻降解藻毒素的关键环境因素；通过研究棕鞭毛虫降解藻毒素能力是否可诱导、降解过程定位分析等，明确棕鞭毛虫对藻毒素的降解策略；利用多组学研究揭示棕鞭毛虫降解藻毒素在转录、蛋白到代谢水平的差异性，确定棕鞭毛虫参与藻毒素降解的关键调控基因、蛋白、代谢物，探讨藻毒素降解的分子调控网络。通过本项目研究，将全面揭示混合营养型原生生物降解藻毒素的分子机制，并深刻认识混合营养型棕鞭毛虫与微囊藻之间的深层次种间关系。

Cyanobacteria blooms represented by toxic Microcystis outbreak frequently with greatly harmful influences on aquatic ecosystem. Mixotrophic protozoa Ochromonas can eliminate toxic Microcystis and degrade microcystins efficiently, therefore dredging the energy channels along food chains. However, how the Ochromonas eliminating toxic Microcystis responds to diversified ecological environments, and how the microcystins are degraded, remain unclear. The present project will expose the Ochromonas degrading microcystins to various abiotic and biotic environments, aiming to the key variables affecting the Ochromonas eliminating toxic Microcystis and degrading microcystins; reveal the degradation strategy of Ochromonas dealing with microcystins via the studies about whether the ability of Ochromonas degrading microcystins is inducible, degradation process location and so on. In addition, the variations in transcription, protein and metabolism levels in Ochromonas while degrading microcystins will be analyzed via the combination of multi-omics study, with the key genes, proteins, metabolites and molecular network for the microcystins degradation determined. The project will fully disclose microcystins degradation mechanism by mixotrophic protozoa, and will benefit the comprehensive understanding of interspecies relationship between Ochromonas and Microcystis.