转移是导致宫颈癌患者治疗失败和死亡的主要原因，淋巴转移是宫颈癌的主要转移途径，而淋巴转移对宫颈癌患者预后不佳。本课题从肿瘤细胞和淋巴管内皮细胞的内在联系入手,为揭示宫颈癌的淋巴转移机制寻找新靶点，提供新思路。Wnt2B是一个糖蛋白信号分子，已被证实与多种肿瘤发生发展及转移相关，但对其在淋巴管转移的具体作用机制还不清楚；我们前期工作中检测到宫颈癌细胞株和组织中均有Wnt2B信号通路分子的表达，多项体内外研究提示其表达水平与淋巴管生成相关。在此基础上，本项目将采用慢病毒表达系统将Wnt2B 和Wnt2B-SiRNA 分别转染宫颈癌细胞中，验证宫颈癌中Wnt2B是否存在自我分泌方式，通过调控其下游靶基因的表达，调控 EMT 而促进转移；另外，验证Wnt2B信号通路在宫颈癌中是否通过旁分泌方式作用瘤周间质细胞，促进瘤周淋巴管生成和淋巴道转移，以及阻断Wnt通路是否有效抑制宫颈癌转移。

Metastasis is the main reason for treatment failure and death in cervical cancer patients. Lymphatic metastasis is the main metastasis pathways in cervical cancer and lymph node metastasis with poor prognosis in cervical cancer patients. This study from the inner link tumor cells with lymphatic endothelial cellsz and provides new mechanism of cervical cancer lymphatic metastasis. Wnt2B is a glycoprotein signaling molecule, which has been confirmed that associated with development and metastasis in a wide variety of tumor.But its role in mechanism lymphatic metastasis of specific is not clear. It were detected the expression of Wnt2B in cervical cancer cell lines and tissues in our preliminary work. Meanwhile several experiment showed that the level of expression of wnt2b is associated with Lymphangiogenesis in vivo and in vitro. This project will adopt the lentivirus expression system to transfect wnt2B and Wnt2B-SiRNA to cervical cancer cells. And verifying whether exists the wnt2B autocrine and throught regulating the expression of its downstream target genes to regulate EMT and promote metastasis in cervical cancer. In addition, validate wnt2B whether by paracrine signaling pathways acting on the tumor stroma cells in cervical cancer and to promote lymph vessels and lymph metastasis. Whether block the Wnt pathway will restrain cervical cancer metastasis effectively.