Bt基因导入影响玉米地上部和地下部诱导防御反应间相互作用的机制研究

Bt基因导入如何影响玉米地上部和地下部诱导防御反应间相互作用是转Bt基因玉米生态安全评价的重要内容之一，而其内在机制一直是国内外研究的焦点，但至今尚未清晰。本项目以不同转化事件的Bt玉米5422Bt1（Bt11）和5422CBCL（Mon810），及其常规近等基因系玉米5422为材料，分别利用茉莉酸和水杨酸模拟虫害和病害作用于玉米的地上部（或地下部），检测处理部位叶片（或根系）和非处理部位根系（或叶片）中Bt蛋白含量、防御相关保护酶活性、直接防御物质丁布和总酚含量及其合成调控基因、直接防御蛋白调控基因以及间接防御挥发物调控基因的变化，比较Bt玉米及其同源常规玉米在其地上部（或地下部）直接诱导效应或地下部（或地上部）间接诱导效应之间的异同，阐明Bt基因导入对玉米地上部和地下部诱导防御反应间相互作用的影响，揭示相关生理生化及分子机制，旨在为科学全面地开展转基因玉米生态安全评价提供重要理论依据。

The effect of Bt(Bacillus thuringiensis) gene introduction on induced defense responses between aboveground and belowground parts of corn has been a key to the ecological safety assessment in transgenic Bt corn. The underlying mechanism is a hotspot both at home and abroad, but it is still unclear. In this project, Bt corns 5422Bt1(Bt11) and 5422CBCL(Mon810) from two transformation events, together with their conventional corn 5422, were used. Damages by insect pest and disease were simulated by applying jasmonic acid and salicylic acid to the aboveground (or belowground) parts of corn. Parameters measured in the treated leaves (or roots) including Bt protein content, activities of defense related protection enzymes, direct defense substances DIMBOA and total phenolic contents and their synthesis mediated genes, direct defense protein mediated genes and indirect defense volatile mediated genes. The similarities and differences in direct defense of aboveground (or belowground) parts, or indirect defense of belowground (or aboveground) parts of corns were compared; the effect of Bt gene introduction on interactions of aboveground and belowground induced defense responses of corn were clarified; the possible physiochemical and molecular mechanisms are revealed. The results can provide an important base for ecological safety assessment in transgenic Bt corn.