ATP合成抑制所致能量亏损在采后龙眼果肉自溶发生中的作用

龙眼是中国南方著名的亚热带特色果品，采后易发生果肉自溶等品质劣变问题，严重影响龙眼果实贮运和销售。前人研究表明，采后果实品质劣变与能量亏损有关；ATP合成抑制剂（二环己基碳二亚胺，DCC）会阻止ATP合成而导致能量亏损；而ATP合成促进剂（琥珀酸钠，DS）则可以促进ATP合成。我们最近的研究发现，ATP合成抑制剂DCC会促进采后龙眼果肉自溶发生，而ATP合成促进剂DS则能延迟采后龙眼果肉自溶发生，但其作用机制尚不清楚。本项目拟研究外源DCC和DS对龙眼果实采后果肉自溶的影响及其与能量代谢、呼吸代谢、活性氧代谢、细胞壁物质代谢的关系，以阐明ATP合成抑制剂（DCC）介导的能量亏损和ATP合成促进剂（DS）介导的能量充足在促进和延迟龙眼果肉自溶发生中的可能作用机制，研究成果可望在阐明通过调控ATP合成所致果实能荷状态改变在调控龙眼采后果肉自溶发生中的可能机制方面提供新的科学依据。

Longan is a famous subtropical fruit in southern China. However, postharvest longan fruit is prone to quality deterioration such as pulp breakdown, which seriously affects the storage, transportation and market of harvested longan fruit. Prior investigations indicate that the quality deterioration of harvested fruit is connected with energy shortage. ATP synthesis inhibitor such as N,N'-Dicyclohexylcarbodiimide (DCC) could suppress the synthesis of ATP and lead to the shortage of energy. Whereas, ATP synthesis accelerator like disodium succinate (DS) could stimulate the synthesis of ATP. Recently, the works of our investigations show that ATP synthesis inhibitor such as DCC could boost pulp breakdown development of harvested longan fruit, but ATP synthesis accelerator like DS could retard pulp breakdown development of postharvest longan fruit. However, the mechanism of pulp breakdown development in postharvest longan fruit caused by DCC-mediated the shortage of energy is still unclear. Thus, the aims of this project are to study the effects of exogenous DCC and DS on the breakdown development in pulp of harvested fresh longan fruit and its relationship with energy metabolism, respiratory metabolism, active oxygen metabolism and cell wall material metabolism, and to elucidate the possible mechanism of the role of ATP synthesis inhibitor like DCC-mediated energy shortage in promoting pulp breakdown development of harvested fresh longan fruit, as well as to clarify the probable mechanism of the role of ATP synthesis accelerator like DS-mediated sufficient energy in retarding breakdown development in pulp of postharvest longan fruit. The outcomes of this project are expected to supply a new scientific basis for clarifying the probable mechanism of the changes in energy status caused by the regulation of ATP synthesis, thereby regulating breakdown development in pulp of postharvest longan fruit.