肠道微生物介导烯啶虫胺抑制意大利蜜蜂（Apis mellifera L．）取食行为的机制

新烟碱类杀虫剂对蜜蜂（Apis mellifera）取食行为具有影响，而其机制尚不明确。申请者前期研究发现，新烟碱类杀虫剂烯啶虫胺不仅显著抑制蜜蜂取食行为，且显著干扰其肠道菌属的相对丰度。鉴于肠道微生物在取食行为中的重要调控作用，本项目拟选取意大利蜜蜂（Apis mellifera L.）工蜂为受试对象，通过恢复差异肠道菌属，研究差异肠道菌属与取食行为的相关性，明确介导烯啶虫胺抑制取食行为的关键肠道菌属；利用代谢组学技术，结合靶向检测法和神经肽组学技术，解析烯啶虫胺对蜜蜂肠道代谢产物、脑内血淋巴和肠道神经递质及脑部神经肽含量的影响，探明肠道微生物介导烯啶虫胺抑制取食行为的途径和调节因子，进而揭示肠道微生物介导烯啶虫胺抑制蜜蜂取食行为的机制。该研究将为新烟碱类杀虫剂对蜜蜂危害的机制提供理论支持，为科学控制新烟碱类杀虫剂对蜜蜂的生态风险提供新思路。

Neonicotinoid pesticides affect the feeding behavior of honeybee (Apis mellifera). But the mechanism is still unclear. In the previous study, the applicant found that nitenpyram, a neonicotinoid insecticide, not only significantly inhibited the feeding behavior of worker bees, but also affected the relative abundance of gut microbiota. Given the gut microbiota playing important role in regulating the feeding behavior, this project plans to select Apis mellifera L. as the subject organism, and through recovering the different intestinal bacteria, the correlation between different intestinal bacteria and feeding behavior will be study and the key intestinal bacteria genus that mediates nitenpyram to inhibit feeding behavior will be identified. Using the metabonomics technology, combined with targeted detection and neuropeptidomics, the content changes of the intestinal metabolic product, neurotransmitter in the hemolymph of brain and intestinal and the brain neuropeptide will be studied, to determine the pathway and regulation factors of gut microbiota-mediated nitenpyram inhibition of feeding behavior, and then will reveal the related mechanism. This study will provide data support for the mechanism of neonicotinoids damage to bees, and provide new ideas for scientific control of the ecological risks of neonicotinoids insecticides to bees.