阳离子聚合物非病毒基因载体作为基因治疗攻克遗传疾病和癌症的新模式，是目前极力发展的载体之一。阳离子聚合物基因载体除了低转染效率和高细胞毒性限制其临床应用外，大多数阳离子聚合物还缺乏自发荧光特性，不能实时示踪基因传递过程和细胞内定位情况。基于以巯基和双键的Michael加成聚合制备自发荧光的叔胺聚合物存在单体需多步繁琐制备、易交联的问题，我们的策略是以简易、高效方法设计响应性的叔胺生色团聚合物，利用该聚合物的自身荧光和阳离子特性构建可示踪的基因载体系统，利用响应性降低毒性和释放基因。本项目拟采用有机膦腈碱（t-BuP2）催化三乙醇胺和双丙烯酸酯类单体经Oxa-Michael加成聚合一步简易制备一系列主链含脂肪族叔胺的响应性超支化聚合物，研究聚合物的荧光和基因转染特性。通过调节平衡聚合物结构与荧光、细胞毒性、转染效率之间的关系，得到低毒、高效、可荧光示踪的基因载体材料。

Cationic polymer nonviral gene vector as a new model of gene therapy to treat human genetic diseases and cancer diseases, which is one vigorously developed of delivery systems. Besides low transfection efficiency and high cytotoxicity, cationic polymer gene vector system does not trace gene delivery process and intracellular location in real time due to the lack of photoluminescent property of cationic polymers. Based on the preparation of polymers with aliphatic tertiary amine chromophore via Michael addition polymerization of thiol and diacrylate monomers have some problems, such as multiple steps of monomer and easy cross-linking of polymerization. Our strategy is to design the efficient photoluminescent cationic polymers with sensitivity for controlled and tractable gene carrier system through simple and efficient method. The responsiveness will to reduce toxicity and control gene release. In this work, a series of the responsive hyperbranched polymers with aliphatic tertiary amine chromophore were synthesized in one-pot by phosphazene base (t-BuP2) catalyzed Oxa-Michael addition copolymerization of triethanolamine and diacrylate monomers. That is, we will investigate the fluorescence and gene carrier properties of polymers containing tertiary amine group. Through to research the relationship between the structure of the polymers and fluorescent properties, cell toxicity, transfection efficiency, the fluorescence traceable cationic polymer gene delivery with low cytotoxicity and high transfection efficiency will be obtained.