水稻SUPER WOMAN 3 (SPW3) 基因调控花器官发育的分子机制研究

水稻是我国重要的粮食作物，其产量的高低直接关系到我国粮食安全等问题，而花器官的发育直接关系到籽粒的形成，相关研究具有重要意义。然而，目前针对水稻花器官发育的研究多集中在染色质和转录水平的基因调控，人们对其发育调控的分子遗传机制的了解远没有拟南芥等双子叶植物深入。先前研究中，我们获得了一个水稻超级雌蕊化突变体，命名为 superwomen3 (spw3)。该突变体的浆片伸长，雄蕊器官同源异形转化为柱头或子房状等异位雌蕊状组织，护颖及內稃特征改变。图位克隆发现SPW3基因编码脱氧羧脯氨赖氨酸羟化酶 (DOHH)，是活化真核翻译起始因子5A (eIF5A) 的关键酶。推测SPW3 可能通过转录后或翻译水平调控水稻花器官发育。目前，相关研究还鲜见报道。本项目中，我们将深入研究SPW3在水稻花器官发育调控网络中的功能，进一步增加人们对水稻花器官发育调控分子机制的认识。

Rice (Oryza sativa L.) is one of the most important grain crops in China. The yield of rice is directly related to the food security of our country. Development of floral organ greatly affects the formation of rice breed. So, it is of far reaching importance to carry out correlation studies. However, most studies on floral organ development in rice focus on gene regulation at the transcription level and the chromatin level which leads to the molecular mechanism of floral organ development in rice is far less understood than that in dicotyledons such as Arabidopsis. In previous study, we identified a severe pistilloid mutant named superwoman 3 (spw3). The lodicule and stamen of spw3 were homeotic transformed into glume-like tissues and ectopic pistil-like tissues, and the characteristics of sterile lemma and palea were changed. SPW3 gene encodes a deoxyhypusine hydroxylase (DOHH), which catalyzes the activation of eukaryotic translation initiation factor (eIF5A). It is speculated that SPW3 may regulate the development of floral organs at the post-transcript level or translation level. But up to now, there is no comprehensive study result to report the mechanism of eIF5A regulating the flower organ identity in rice at home and abroad. In this project, the molecular function of SPW3 gene in the regulation network of rice flower organ development is expected to be uncovered in-detail by applying various technologies or methods.