LsMYBs转录因子调控换锦花红蓝复色花形成的分子机制

花色素苷是决定植物花色的重要成分，R2R3-MYBs类转录因子可调控花色素苷生物合成，复色花形成的作用机理复杂，探讨复色花形成调控机制具有重要的意义。石蒜属植物换锦花具有独特的红蓝复色花表型。本项目选用课题组拥有的各种红蓝复色花变异表型的换锦花种质为试材，以前期研究发现的可能影响换锦花花色表型的LsMYBs（LsMYB1、LsMYB4和LsMYB5）转录因子为切入点，采用qRT-PCR、RNA荧光原位杂交技术、酵母单杂交技术、换锦花花瓣瞬时表达技术、凝胶阻滞实验和DNase I足迹体外试验以及异源瞬时转化矮牵牛花瓣等分子生物学、功能基因组学及生理生化等研究手段，聚焦于破解LsMYBs对红蓝复色花形成调控的分子机制，有望获得有关植物复色花形成及调控网络的新知识以及可用于花色基因工程遗传改良的分子靶标等DNA元件，为石蒜属植物及更多观赏植物复色花的人工创制和分子育种提供理论基础。

Anthocyanin is an important plant pigment and the biosynthesis of anthocyanin can be regulated by R2R3-MYBs transcription factors. However, the formation mechanism of multiple colored flowers is complex. Therefore, it is of great significance to explore the formation mechanism of multiple colored flowers. L. sprengeri (Lycoris genus) has unique flower pattern with red and blue color on petals. But the formation mechanism of multiple color flowers is yet to know. In this study, we will use L. sprengeri multiple color flowers as our materials. Preliminary data indicated that the transcription factors LsMYBs ( LsMYB1, LsMYB4 and LsMYB5 ) possibly affected the flower color phenotypes in our previous research. The molecular regulatory mechanism of multiple color flowers will be further studied by our group using molecular biology, function genomics, and physiological and biochemical research methods like qRT-PCR, RNA fluorescence in situ hybridization, yeast one hybrid, L.sprengeri petal transient expression technique, electrophoretic mobility shift assay (EMSA), DNase I footprint and allogenic transformation in petunia. The results are expected to reveal the plant multiple color flowers formation and regulatory network and molecular targets DNA elements for genetic improvement of flower color through genes engineering methods, which will provide a theoretical basis for artificial creation and molecular breeding of multiple color flowers in Lycoris species and ornamental plants.