BR信号和光周期通过BIN2-BES1-ELF3分子模块协同调控拟南芥花期的分子机制

花期是关键的农艺性状，对植物产量和品质有着重要影响，也是目前植物发育生物学研究的热点。促生长激素油菜素内酯（BR）在调控植物生长发育和响应逆境胁迫信号过程中有重要作用，然而，BR调控植物由营养生长向生殖生长转变的作用机理知之甚少，因此阐明BR如何协同光信号精准调控花期的分子机制，将为农作物产量提供重要的理论指导。项目前期结果表明：BR信号在BIN2和BES1两个关键节点参于花期调控，BIN2负调控而BES1正调控花期；通过蛋白互作筛选发现BIN2和BES1都能与早花基因ELF3互作，在此基础上，通过分子生物学和生物化学手段，建立BIN2介导BR信号和光周期信号在精准调控花期方面的调控模型；另外，通过高通量测序和生物化学手段解析BES1介导BR信号与光周期信号交叉互作的分子机制，研究结果为将来提高农作物产量提供理论指导。

Flowering is a crucial agronomic trait, which has an important effect on plant yield and quality. The research of flowering is also important for plant developmental biology. Brassinosteroids (BRs), as the major growth-promoting hormones, plays a key role in regulating plant growth and in response to stresses stimulus. However, little is known about the mechanism of BR involved in the vegetative to reproductive transition. Thus, to elucidate the molecular mechanism of how BR coordinates with photoperiods to accurately regulate flowering is essential to increase crop yields. Our preliminary results indicated that the BR signaling is involved in the regulation of flowering at the key points of BIN2 and BES1, BES1 positively regulated the flowering but BIN2 in reverse. Besides, by screening protein-protein interaction, it was found that BIN2 or BES1 can both interact with ELF3, which is a flowering-promoting gene. Therefore, our study plan to establish a regulation mode of BIN2-mediated BR signaling and photoperiod in regulating flowering by molecular biology and biochemistry methods. In addition, we will also explore the molecular basis of the cross-interaction of BES1 mediated BR signaingl and photoperiod by high-throughput sequencing and biochemistry methods. Our data will provide some clues to the theoretical guidance for further improvement of crop yield.