接种疫苗是水产养殖业健康可持续发展的关键支撑技术。各种新型水产疫苗的研究和开发正以前所未有的速度发展着。这些发展是以对疫苗的各种分子机制的了解为基础的，这将使得疫苗的研发设计更具合理性和科学性。冻干剂型作为水产活疫苗的重要产品形式和研发内容面临着许多挑战，疫苗细胞的冻干耐受稳定性是其中最为突出的挑战之一。积累相容性溶质是细菌普遍采用的一种应对环境胁迫的细胞保护策略。积累合适的相容性溶质可为微生物细胞在低温胁迫下提供有效地保护。对大多数微生物较为有效的相容性溶质为海藻糖、甜菜碱和四氢嘧啶等。本研究以海洋鱼类鳗弧菌活疫苗为研究对象，旨在发现存在于鳗弧菌中的相容性溶质生物合成和摄取相关功能基因，阐明相容性溶质胞内积累的冷胁迫响应机制，并以此分子机制为基础，建立起基于相容性溶质胞内积累下的鳗弧菌活疫苗冻干耐受保护策略，为开发鳗弧菌活疫苗冻干剂型提供全新的优化策略提供理论支持。

Vaccination is the key supporting technologies for the health and sustainable development of aquaculture.Research and development of vairous new type aquatic vaccines are being developed at an unprecedented rate.The development is based on understanding of molecular mechanisms of vaccine.This will enable design and development of the vaccine is more reasonable and scientific.Freeze-drying as an important production mode of aquatic live vaccine and R & Dcontent is facing many challenges.Freeze-drying tolerance and stability of vaccine cell is one of the most prominent one of the challenges.Accumulation of compatible solutes is a strategy widely employed by bacteria to achieve cellular protection against environmental stresses.Bacteria use compatible solutes to protect their cytoplasmic biomolecules against cold stress.Much studies have revealed that the preferred compatible solutes for the majority of bacteria are betaine, ectoine and trehalose.In this study, the we will aim at identifying the compatible solutes biosynthesis and uptake relevant functional genes in Vibrio anguillarum,and find the mechaisms of accumulation of the compatible solutes in cell agansit cold stress. Based on this mechaism, we will establish regulation mechanism for freeze-drying tolerance of live Vibrio anguillarum vaccine by accumulation of compatible solutes,and to provide a new optimization strategy for the development of more stable freeze-dried live Vibrio anguillarum vaccine.