卵巢早衰（POF）系多种病因所致，发生于青春发育后至40岁前妇女自然出现卵巢功能的衰退，导致性器官萎缩和闭经，内分泌激素异常，甚至不孕，并伴随其他内分泌自身免疫疾患，此病病因复杂，故其治疗较困难，常用性激素替代疗法，但不能恢复卵巢周期性功能，并有明显副作用。临床研究表明中医药对POF后性激素调节和不孕的治疗有肯定疗效，然而药物作用机制尚不明确。我们的前期研究集中在卵巢形态学和卵泡凋亡两个方面。本研究拟在前期研究的基础上，结合细胞移植技术和细胞微囊化技术，通过研究补肾活血方干预微囊化卵巢颗粒细胞异体移植对去势大鼠卵巢颗粒细胞芳香化酶及其TGFβ/Smad信号转导途径的调控作用,探讨血清中雌激素水平及子宫组织中雌激素受体变化趋势的相关性，进而明确芳香化酶促进颗粒细胞雌激素生成的作用及其机制，探索补肾活血方促进颗粒细胞芳香化酶活性作用靶点，为POF患者寻找一种新的治疗方法提供理论基础及实验依据。

Premature ovarian failure(POF)is caused by a variety of causes of ovarian failure,happen from puberty development to 40 years previously for women,and leads to sexual organ atrophy,amenorrhea,abnormal endocrine hormones,even infertility,often with other endocrine autoimmune disorders.The etiology of POF is very complex,so the treatment is more difficult,commonly used hormone replacement therapy,but not to restore ovarian periodic function,and have significant side effects. Clinucal studies have shown that Traditional Chinese medical（TCM）has positive effect on sex hormone regulation and infetility treatment in POF,but the mechanism of drug action is unclear.Our previous study focused on ovarian morphology and follicular apoptosis.This research is based on previous study, combined with cell transplantation and cell microencapsulation technique to study the regulatory effect of microencapsulated ovarian granulosa cells on ovarina aromatase activity and TGFβ/Smad signal transduction inovarectomized rats after allogenetic transplantation through Bushenhuoxue decoction intervene.to explore the correlation between serum estrogen levels and the change in the estrogen receptor ,and then clarify the mechanism that aromatase promote the proliferation of GC,investigate a target where Bushenhuoxue decoction promote the aromatase activity,and looking for a new treatment method to the POF patients to provide theoretical basis and experimental evidence.