如何积极有效促进多巴胺能神经元功能恢复，是防治PD的关键。我们前期研究证明电针可调控自噬、促进线粒体功能恢复。研究表明，PINK1/Parkin介导的线粒体自噬在PD发病中至关重要。那么电针能否通过PINK1/Parkin通路调控线粒体自噬和线粒体质量发挥神经元保护作用？基于此，本项目拟通过观察电针对线粒体超微结构、自噬体、自噬溶酶体、线粒体质量等影响，揭示电针可能通过启动线粒体自噬、调控线粒体质量，维持细胞稳态；通过观察电针对自噬、PINK1/Parkin通路相关蛋白及分裂/融合相关蛋白及其mRNA表达，明确电针可能通过PINK1/Parkin通路调控线粒体自噬和线粒体质量控制；通过自噬诱导剂、阻滞剂和Parkin基因敲除鼠的应用，验证“电针通过PINK1/Parkin通路促进线粒体自噬、调节线粒体分裂/融合，调控线粒体质量，维持线粒体稳态，保护多巴胺能神经元功能”这一科学假说。

How to actively and effectively promote the functional recovery of dopaminergic neurons is the key to the prevention and treatment of PD. Our previous studies have shown that electroacupuncture can regulate autophagy and promote the recovery of mitochondrial function.Recent studies have shown that PINK1/ Parkin-dependent mitophagy plays an important role in the pathogenesis of PD.Then whether electroacupuncture can regulate mitochondrial autophagy and mitochondrial quality through PINK1 / Parkin pathway to exert the neuroprotective effect of electroacupuncture?Based on this，this project intends to observe the effects of electroacupuncture on mitochondrial ultrastructure, autophagosomes, autophagy lysosomes and mitochondrial quality, revealing that electroacupuncture may maintain cell homeostasis by activating mitophagy and regulate mitochondrial quality. by observing the effects of electroacupuncture on mitophagy,PINK1 / Parkin pathway-related proteins and their mitotic / fusion related proteins and their mRNA expression, we found that electroacupuncture may regulate mitochondrial autophagy and mitochondrial quality control through the PINK1 / Parkin pathway.By applying autophagy inducers, blockers and Parkin knockout mice,verifies the scientific hypothesis that “Electroacupuncture promotes mitophagy, regulates mitochondrial division / fusion, regulates mitochondrial quality, maintains mitochondrial homeostasis, and protects dopaminergic neurons through the PINK1 / Parkin pathway”.