物种形成的分子机制研究是进化生物学领域最为引人关注的课题之一，特别是近年来随着高通量测序技术的运用，从全基因组水平鉴定在物种适应性分化过程中与表型和生理性状相关的基因已成为目前的研究热点。本项目拟对分布于不同生境中的两个生态物种长白耧斗菜（Aquilegia japonica）与尖萼耧斗菜（A. oxysepala）进行全基因组重测序（whole genome resequencing）与甲基化组（methylome）测序。基于所获得的分子与形态学数据，我们拟对物种内遗传多样性较低的区域以及物种间遗传/表观遗传分化较大的区域进行比较基因组学与表观基因组学分析，以期望通过本项目的研究鉴定在适应性分化过程中起到关键作用的基因，并阐述其改变这两个生态物种表型与生理性状的遗传与表观遗传机制。

The molecular mechanism of speciation is one of the most important issues in the evolutionary biology. In recent years, the applications of high-throughput sequencing techniques provide us opportunities to identify the key genes that affect morphological and physiological traits. In this study, we plan to sequence both the genomes and epigenomes of two ecological species Aquileiga japonica and A. oxysepala. The aims of this study are to identify the low genetic diversity genomic regions within species and high genetic/epigenetic differentiation genomic regions between species. Thereafter, we will used both genomic and phenotypic data to demonstrate how and to what extend these genes affect the morphological and physiological traits of the two ecologcial species through comparative genomics and epigenomics analyses.