经典分子生物学强调“假设驱动”分子机理的基础研究，近年来兴起的合成生物学强调“工程化”创建人造简约生命系统。在本申请项目中，我们将利用前期工程化创建的单条线型染色体的酿酒酵母SY14和单条环型染色体的酿酒酵母SY15为研究材料，针对SY14和SY15与野生型相比较呈现的许多出乎意料的有趣的新现象，借鉴经典分子生物学 “假设驱动”基础研究.和借鉴合成生物学“工程化”理念，围绕三个科学问题，尝试突破“点着丝粒”与“区域着丝粒”、“复杂真核端粒”与“简约原核端粒”、“线型染色体”与“环型染色体”的自然界限。通过 “人造”新的简约生命系统，为从新的角度、新的途径深入理解分子遗传系统的基本现象打开新的窗口,同时为解决真核生物染色体畸变的重大应用问题开辟新的道路。

The class molecular biology emphasizes “hypothesis-driven” basic researches on molecular mechanisms. The synthetic biology was newly found with its “engineering” feature. In this application, we utilize the two created Saccharomyces cerevisiae strains SY14 and SY15, which contain a single linear and circular chromosomes, respectively. Comparison to wild-type, SY14 and SY15 cells display many interesting phenotypes. We will combine “hypothesis-driven” and “engineering” thoughts to address three relative scientific questions. We are trying to break the boundaries of natural life, including point centromere vs. region centromere, eukaryotic telomere vs. prokaryotic phage telomere, linear chromosome vs circular chromosome. These investigations featured both “engineering” and “hypothesis” may provide a new way to address some basic biological questions, and may provide a solution for eukaryotic circular chromosome.