类风湿关节炎（RA）“炎症极化”可分泌促炎性细胞因子，加速机体炎症反应，加重关节病变。RA免疫炎症与环RNA (circRNA)、微小RNA(miRNA)及Notch通路激活有关。前期研究表明，circ-CBLB是RA免疫炎症关键circRNA，miR-155-3p、EST-1是其下游靶基因，参与Notch通路激活和炎症极化过程。“健脾化湿通络”方新风胶囊能调节Notch通路，调控炎症极化平衡，改善RA关节症状。但新风胶囊调节炎症极化机制不清。本课题组提出新风胶囊通过调节circ-CBLB、miR-155-3p表达，促进EST-1分泌，抑制Notch通路过激活，调控炎症极化，抑制RA免疫炎症反应。为验证以上假说，本研究采用RNA pull down、荧光素酶报告实验、RIP、qPCR、免疫荧光、流式细胞术等方法，观察新风胶囊调控炎症极化平衡机制。本研究将为中医药治疗RA提供依据和途径。

Rheumatoid arthritis (RA) "inflammatory polarization" can secrete pro-inflammatory cytokines, accelerate the body's inflammatory response, and aggravate joint disease. RA immune inflammation is associated with activation of circular RNA (circRNA), microRNA (miRNA), and Notch pathway. Previous studies have shown that circ-CBLB is a key circRNA for RA immune inflammation, miR-155-3p and EST-1 are downstream target genes involved in the activation of Notch pathway and inflammatory polarization. "Xianpi Huashi Tongluo" Fangxinfeng Capsule can regulate the Notch pathway, regulate the polarization of inflammatory polarization, and improve the symptoms of RA joints. However, the mechanism of Xinfeng Capsule regulating inflammatory polarization is unclear. Our group proposed that Xinfeng Capsule can promote the secretion of EST-1, inhibit the overactivation of Notch pathway, regulate the inflammatory polarization and inhibit the immune inflammatory response of RA by regulating the expression of circ-CBLB and miR-155-3p. In order to verify the above hypothesis, this study used RNA pull down, luciferase reporter assay, RIP, qPCR, immunofluorescence, flow cytometry and other methods to observe the regulation of inflammatory polarization balance mechanism by Xinfeng Capsule. This study will provide evidence and ways for Chinese medicine to treat RA.