嗜盐古菌乙酸合成和激活及其乙酰化调控机制

嗜盐古菌在高盐生态系统的碳元素循环中发挥着极其重要的作用，同时在生物技术的开发应用方面也显示出巨大的潜力。因此，嗜盐古菌复杂且具有独特机制的碳代谢研究是当前微生物学领域的重要研究课题之一。本项目将基于申请人在嗜盐古菌碳代谢领域长期的工作积累，以高效合成聚羟基脂肪酸酯（PHA）的地中海富盐菌为研究对象，从分子水平系统研究嗜盐古菌的乙酸代谢过程，重点揭示嗜盐古菌乙酸合成和激活途径及其关键酶，解析乙酰化修饰对乙酸合成和激活的分子调控机理，同时将所获得的乙酸代谢理论用于地中海富盐菌的代谢工程改造，提高其PHA产量。本项目所取得的研究成果将为认识嗜盐古菌生理生态功能提供新见解，同时对改造嗜盐微生物生产其他功能物质具有重要的指导意义。

Haloarchaea are members of halophile community, found in various hypersaline environments. They play a crucial role in carbon recycling in saline ecosystems. class of microbial resources. Meanwhile, they show great potential in the biotechnology field. Therefore, the research about carbon metabolism of haloarchaea has been one of the important topics in Microbiology. Based on the applicant’s achievement in physiology and metabolism of haloarchaea, this project will systematically study the acetate metabolism in Haloferax mediterranei with strong ability to synthesize a large amount of polyhydroxyalkanoates (PHA) intracellularly. We will reveal the key pathways and enzymes involved in acetate production and activation, and elucidate the regulation of protein acetylation of key enzymes involved in acetate production and activation, as well as, we will use the obtained knowledge to engineer H. mediterranei to reduce its acetate overflow and thus enhance its PHA productivity. The achievements from this project will enrich our knowledge about the physiological and ecological functions of haloarchaea and show beneficial effects on engineering haloarchaea to produce other functional chemicals.