艾纳香DNA甲基化和miRNA对镉胁迫积累的生理响应

艾纳香为一种重要的中草药，在临床上有众多的医用价值。镉(Cd)是生物毒性最强的土壤污染物之一，它极易在植物体内积累，并通过食物链影响动物与人类的健康。因此研究Cd在艾纳香植株体内积累特性就尤为重要。本项目前期研究表明艾纳香对低浓度Cd污染土壤有高富集的作用，且随Cd浓度的升高其积累量增加。由于目前还未见有关于艾纳香Cd胁迫积累特性的相关深入报道，为此本项目设置了3个Cd浓度处理(0, 0.5和5.0mg/kg)，计划从Cd胁迫积累下植株根系分化、金属离子转运、离子区室化、螯合作用、抗氧化防御、细胞壁合成与防御、信号传导方面进行生理响应研究，再结合相应器官DNA甲基化和miRNA数据分析以获得植株地上部和地下部对Cd响应的差异性和对低浓度Cd高富集的生理机制。

Blumea balsamifera is the important Chinese medicinal herb, and has many medical values in clinic. Cadmium(Cd) is the most toxic one of the soil pollutants towards biology, which can easily be accumulated in plants, and affect the health of animals and humans through the food chain. Therefore, it is particularly important to study the accumulation characteristics of cadmium in plants of Blumea balsamifera. The preliminary studies showed that Blumea balsamifera had the high Cd enrichment effect on the soil polluted with low concentration of Cd, its accumulation increased with the increase of Cd concentration. Due to the lack of in-depth reports on Cd accumulation characteristics of Blumea balsamifera under Cd stress, the experiments will be designed to study the physiological responses of Blumea balsamifera on the root differentiation, metal ion transportation, metal ion compartment, sequestration, antioxidant defense, cell wall synthesis and defense, signal transduction under Cd stress at three different concentration (0, 0.5 and 5.0mg/kg) in the present project. Combined with the above results, the physiological mechanisms of high Cd enrichment of plants at low soil Cd concentration and responsive differentiation of the shoot and the root to the soil Cd in Blumea balsamifera will be clarified with the analytical data of DNA methylation and miRNA of the corresponding organ.