本项目从病原体分子快速高敏现场测量出发，具体针对几种重要病原体，提出基于FEN1酶新型探针引物的高分辨数字微流控芯片研究，实现病原体测量的快速（≦15min）、高敏 (单拷贝/样本)、简便（现场直接测量），建立病原体核酸测量新系统，突破当前常规分子恒温测量的技术瓶颈并解决背后基础科学问题（FEN1酶链置换剪切效应、数字液滴内酶促反应动力学研究，锁核酸结合动力学等）。最终建立更加普及化和亲民化的病原体分子现场POCT测量系统，并实现新技术的产学研转化，应用于病原体现场主动预警和防控。

Since infectious diseases are characterized by rapid onset and wide spread among people, rapid and accurate identification of infectious pathogens is of great clinical value for the prevention,control and early-treatment of such diseases. This project aims to the development of a novel rapid (≦ 15 min) , sensitive (single copy) , specific and simple detection method based on the FEN1 enzyme probe-primer and high resolution digital microfluidics. Meanwhile, we perform the study of the basic scientific problems, including FEN1 enzyme-based probe primer, the enzymatic reaction kinetics in digital droplets.