干旱区土壤盐渍化一直是困扰农业和林业生产的一大难题。作为西北盐碱地改良的首选植物之一，宁夏枸杞对盐的耐受性有独特的适应机制。本项目拟采用22Na+同位素示踪技术、非损伤微测技术和激光共聚焦技术研究宁杞1号在NaCl 胁迫条件下，Na+净吸收速率、Na+转运和积累以及Na+在细胞和亚细胞中的分布；同时利用离子通道抑制剂研究枸杞吸收Na+ 的途径；采用分子生物学手段研究驱动因子质膜H+-ATPase、Na+/H+转运蛋白（SOS1）和液泡膜H+-ATPase、Na+/H+反向转运蛋白（NHX1）基因表达在Na+的外排和区隔于液泡中作用。从而在组织、细胞和分子水平阐明宁夏枸杞Na+的吸收途径、积累机理及驱动因子协同作用的分子机制，为盐生植物枸杞耐盐类型的确定和宁夏枸杞耐盐机理的揭示增添新内容。本项目的完成可以为西北地区利用木本盐生植物改良大面积的盐碱地，促进西部特色产业的发展提供技术和理论依据。

Salinity in arid zones is one of the major abiotic factors limiting agricultural and forestry productivity. Ningxia Lycium barbarum L., is one of the valuable plants for improving salt-alkali soil with unique mechanism to adapt salt stress. In this proposal,the sodium absorption and accumulation, as well as the cellular and subcellular distribution of sodium in Ningxia Lycium barbarum L.under NaCl stress, will be investigated, using techniques of 22Na+ chasing, non-invasive micro-test and confocal scanning laser microscopy. The pathways of sodium absorption will also be identified by utilizing various channel inhibitors. The copperative effects of driving forces of PM H+-ATPase, PM Na+/H+antiporter (SOS1), vacuolar H+-ATPase and/or Na+/H+antiporter (NHX1) on the cytoplasmic sodium efflux will be ascertained by determining the transcriptional expression of these genes. We anticipate that this work will provide fundamental information for understanding the mechanisms of sodium absorption and accumulation, and the copperative effects of driving forces of the salt tolerant Ningxia Lycium barbarum L.. This study will also help us to determine the phenotype of salt tolerance for this plant. An accomplishment of this study will provide important information for improving salt-alkali soil by planting salt-tolerant plants, as well as promoting the development of special industry in Western China.