雄性不育的利用在水稻产量提高过程中做出了巨大贡献。大多数雄性不育突变体都是由花药或花粉粒发育异常所导致的，因此相关分子机理的研究对操控水稻育性以及了解雄性生殖发育的分子机理都具有非常重要的意义。水稻PTC1编码一个PHD-finger蛋白，作为一个至关重要的基因表达调控因子参与花药发育过程中。ptc1表现为绒毡层细胞降解延迟以及雄性不育。但作为表观遗传因子之一的PHD-finger蛋白是通过什么深层次的分子机制来调节基因表达，以及是否与组蛋白相互作用来调控基因表达，这些问题目前都不清楚。本项目拟以一个ptc1等位突变体（osms1）为研究材料，在探明与OsMS1具有相互作用的组蛋白甲基化类型后，用相应组蛋白特异抗体检测突变体中组蛋白甲基化修饰水平，确定OsMS1所影响的组蛋白修饰；再利用Chip-seq全基因组分析OsMS1影响的组蛋白甲基化情况，深层次阐明雄性生殖发育的分子机理。

The application of rice male sterility plays an important role in the process of rice yield improvement. Most male sterility is caused by the defective anther or pollen development. Therefore, the study about rice anther and pollen development is critical for practical application in rice breeding and understanding the molecular mechanism of male reproductive development. In rice, a PHD-finger protein, encoded by PTC1, is an important gene expression regulator, which plays an important role in anther development. However, the fundamental mechanism of PTC1 is unknown, and whether the PHD-finger protein regulates gene expression by interacting H3 histone protein is also unknown. Previously, we found a spontaneous male sterile mutant osms1, which is allelic to ptc1. In this project, we plan to use Pull Down and CoIP assays to analyze the interaction between OsMS1 and various methylated H3 histone, and then use west blot to analyze the interacted protein level between wild type and osms1, and use Chip-seq to genome-wide analyze the target genes of H3 histones which are interacted with OsMS1 and down-regulated in osms1, and analyze the relationship between down or up-regulated genes and histone methylation. The result will be helpful for understanding the fundamental mechanism of PHD-finger protein OsMS1, and for understanding the gene regulation network of anther development.