在已经取得较好研究进展的基础上，结合野外研究与室内模拟，利用形态学方法研究红树林生态系统中红树根系AMF的侵染率和侵染结构；采用SSU-IST-LSU rDNA长片段序列与400/800bp的短片段rDNA序列相结合的分子标记方法与高通量测序技术研究不同生境中红树根际系统的AMF菌种多样性；系统地开展不同时空条件下红树林生态系统中的AMF生物多样性资源研究。通过筛选、鉴定与扩繁并结合组织化学手段与DNA微阵列技术，研究AMF对红树的生源要素吸收以及对重金属、盐耐受和适应机理的影响。综合比对鉴定出红树对N、P吸收能力高、对盐及重金属耐受与适应能力强的优势AMF菌种；开发高效制备与保存优势AMF菌剂的方法与技术。丰富和完善生态学尤其是红树林根际生态学研究理论；创新湿地AMF菌剂筛选的技术手段，丰富我国AMF种质资源库，为AMF菌剂推广应用以及为红树林生态系统保育恢复提供科学的理论依据和技术支撑

Based on the previous progresses we have made, our research team will further combine field studies with laboratory simulation, using morphological method to identify the arbuscular mycorrhizal fungi (AMF) colonization rate and structure in mangrove roots of typical mangrove ecosystem, and using SSU-ITS-LSU fragment (provided by Sanger sequencing) and shorter fragments (such as the c. 400- or 800-bp reads provided by 454-sequencing) as the molecular markers, as well as the high-throughput sequencing technology to investigate the AMF diversity in mangrove rhizosphere under different living conditions, thus to explore the AMF biodiversity resources in mangrove ecosystems under different spatiotemporal scales. Through screening, indentification and propagation of the AMF strains, combined with histochemistry method and DNA microarray technology, the effect of AMF on the absorption of biogenic elements as well as the tolerance mechanisms of mangrove to heavy metal and salt stress will be studied. After comprehensive comparison, the dominant AMF species which promote the absorption capacity of nitrogen and phosphorus elements and the tolerance ability to heavy metal and salt stress in mangrove will be identified. The efficient method and technology to prepare and preserve the dominant AMF species will be explored. Our results will develop and perfect the theory of ecological research especially the mangrove rhizosphere ecology research, innovate the screening technology of wetland AMF species, enrich Chinese AMF germplasm resources bank, and provide theoretical basis and technical support to the application of AMF agent in large scale as well as the conservation and recovery of mangrove ecosystem.