14-3-3蛋白调控轮枝镰刀菌的菌丝生长和致病性的分子机制

甘蔗梢腐病严重影响甘蔗产量和糖分。甘蔗梢腐病可由多种镰刀菌引起，其中轮枝镰刀菌（Fusarium verticillioides, 简称Fv）是引起我国甘蔗主产区广西的甘蔗梢腐病的主要病原菌。该菌的侵染过程及致病机制尚不清楚。14-3-3蛋白是在真核生物中广泛存在的重要调节蛋白，但在镰刀菌中的调控作用尚不清楚。前期研究发现，在轮枝镰刀菌中有两个编码14-3-3蛋白的基因FvBmh1和FvBmh2，其中FvBmh2主要调节菌丝生长、产孢和致病力。双突变FvBmh1和FvBmh2基因似乎具有致死作用。本研究拟通过构建基因缺失、干扰及过表达突变体，进行FvBmh1和FvBmh2基因表达模式分析及蛋白互作研究，从而揭示14-3-3蛋白在轮枝镰刀菌菌丝营养生长、分生孢子产生和萌发、侵染宿主致病过程中的调控作用机制，为发掘关键靶标、研发绿色防控方法及创制抗梢腐病甘蔗新种质提供指导。

Sugarcane Pokkah boeng disease is a major disease that significantly reduces the sugarcane yield and sugar content. Fusarium verticillioidesis (abbreviated Fv) is the major pathogen causing Pokkah boeng disease in Guangxi, the main sugarcane growing area in China. However, the mechanism of the pathogenicity of the pathogen is not yet understood. 14-3-3 proteins are relatively conservative and have been widely found in fungi, but their function in Fusarium spp. has been reported. In our previous studies, it was found that there are two homologs of 14-3-3 proteins, FvBmh1 and FvBmh2 in Fv and that FvBmh2 played a major role in vegetative mycelium growth, conidiation and virulence. Double deletion of FvBmh1/FvBmh2 seemed to be lethal to Fv. To investigate the role of 14-3-3 proteins in pathogenicity regulation in Fv, we will construct FvBmh1 and FvBmh2 null mutants and combination of null mutant and RNA interference of either genes, and over-expression mutants. By comparison of the gene expression profile of these mutants, and identification of the proteins interacting with 14-3-3 proteins, we shall be able to uncover the biological function of 14-3-3 proteins and the pathways that they are involved in hyphae growth, conidiation, spore germination, and pathogenecity. The knowledge gained in this study will help to identify key cellular targets for better control of the disease and facilitate the development of new sugarcane germplasms resistant to the Pokkah boeng disease.