城市污水内源短程反硝化协同短程硝化强化主流Anammox深度脱氮及作用机制

厌氧氨氧化（Anammox）技术的应用是现今污水生物处理领域研究和工程前沿，但实现较为理想的城市污水主流Anammox仍面临诸多挑战。申请人近期的研究发现内源短程反硝化和短程硝化可实现优势互补，协同提高主流Anammox经济高效脱氮的稳定性。但该系统存在碳氮转化途径复杂、各生化反应间和不同功能菌群间协同竞争关系尚不明确等问题，因此研究其内在作用机制十分必要。.本项目以城市污水为处理对象，在初步建立城市污水内源短程反硝化协同短程硝化强化主流Anammox深度脱氮新工艺的基础上，研究不同条件下污染物碳和氮的迁移转化规律及各生化过程的动力学特征，解析核心功能菌的代谢特征、功能基因表达水平及协同竞争机制，深入揭示该工艺的内在作用机制；进而考察工艺运行的关键影响因素并在非稳态条件下验证，明确该工艺稳定性与菌群结构间的调控关系，建立合理的运行控制策略，最终稳定实现城市污水主流Anammox深度脱氮。

The application of anammox technology is the frontier of research and engineering in the field of biological wastewater treatment. But achieving the ideal mainstream anammox still faces many challenges. Our recent study found that the endogenous partial denitrification (EPD) and partial nitrification (PN) could complement each other's advantages, synergistically improving the stability of mainstream anammox for the economical and efficient nitrogen removal. However, some questions such as complicated carbon and nitrogen transformation pathways, unclear relationships among different biochemical processes and arguable cooperation/competition mechanism among functional bacteria are remained in this system. Therefore, the inherent mechanism of action is required to be further investigated..In this project, municipal wastewater is selected as treatment object. Based on the initial establishment of the novel nitrogen removal process of enhanced mainstream anammox with the cooperation of EPD and PN, the transportation and transformation rule of pollutants (C and N) and the kinetic characteristics of each biochemical process will be studied with the different conditions. Meanwhile, the metabolic characteristics, functional gene levels and cooperation/competition mechanism of the main bacteria will be assessed, deeply revealing the inherent mechanism of action in this process. The key influential factors of this process will be also investigated, thereby which will be verified under the unsteady state conditions. Furthermore, the regulatory relationship between process stability and microbial community structure will be analyzed, and the reasonable operation control strategies will be developed. Finally, the advanced nitrogen removal from municipal wastewater via mainstream anammox will be achieve stably.