糖尿病慢性创面是外科经常遇到的难题，目前在临床上仍缺乏有效的治疗方法。血管病变引起的组织细胞慢性缺血缺氧是其发生的重要原因，但分子机制尚未完全阐明。文献报道和我们前期研究表明，免疫信号蛋白CD100不仅是创面愈合起始阶段的关键分子，并且在体内外都可诱导强有力的血管新生。我们前期初步临床研究中发现，糖尿病足溃疡组织中CD100表达较急性创面显著降低，但CD100表达下降是否和糖尿病足患者病情相关，补充外源CD100能否通过促进血管生成而促进创面愈合还不清楚。本课题拟在前期基础上扩大糖尿病足患者样本的收集，分析CD100及其受体表达同病情、病程及预后的关系；利用糖尿病小鼠慢性创面模型及缺氧高糖内皮细胞培养模型，通过加入外源CD100及调节受体信号，探讨其对创面愈合进程、血管生成作用及内皮细胞功能的影响，目的是阐明CD100参与糖尿病慢性创面愈合的作用及机制，为糖尿病慢性创面药物治疗探索新靶点。

Diabetic chronic wound is demonstrated to be an increasing public-health problem nowadays. Unfortunately, treatment provided for diabetic chronic wound is often inadequate. Many evidences indicate that the chronic ischemia and hypoxia condition resulting from the impaired microcirculation correlated well with the pathogenesis of chronic wounds, however, the molecular mechanisms involved have not been fully elucidated. CD100, a kind of immune semaphorin, was demonstrated to play an important role in normal wound healing process. Our previous studies and researches in other groups showed that CD100 could promote effective angiogenesis not only in vivo but also in vitro. In our previous preliminary clinical studies, CD100 expression in diabetic foot ulcers was significantly lower than that in acute wounds. But it was still unknown that whether the CD100 expression correlated with the pathogenesis of diabetic foot ulcer and whether the addition of exogenous soluble CD100 could accelerate chronic wound healing by promoting angiogenesis. Based on our previous studies, this research intends to expand the diabetic foot sample collection and analyze the relationship between the expression of CD100 and its receptor and the wound healing course and the disease prognosis. The diabetic mouse chronic wound healing model and the hypoxia and high glucose endothelial culture model would also be treated with exogenous soluble CD100 or the CD100 receptor blocking reagents for regulating CD100 and its receptor signals, and then the impact of different treatment on wound healing process, angiogenesis and endothelial function will be evaluated. Through this research, the role and mechanism of CD100 and its receptor involving in chronic wound healing process would be confirmed, the results might provide potential new therapeutic target to chronic wounds healing.